Li Yi, Hu Hong-Ying, Wu Qian-Yuan, Yang Hai-Yang
ESPC State Key Joint Laboratory, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Huan Jing Ke Xue. 2007 Apr;28(4):902-7.
Seven bacterial strains that can degrade nitrobenzene at low temperature were isolated from the sediments of a nitrobenzene polluted river. One of the strains, NB1, can mineralize 20 mg/L nitrobenzene completely under the temperature range from 2.5 degrees C to 35 degrees C with an optimum temperature of 25 degrees C. At 5 degrees C, the strain can grow and degrade 20 mg/L nitrobenzene under pH 6 - 9. As long as the concentration of nitrobenzene was not higher than 100 mg/L, it can be degraded by the strain successfully. The strain was identified as Pseudomonas putida according to its morphology, biochemical properties and 16S rDNA sequence analysis. The growth and nitrobenzene degradation character of the strain at different temperature, especially at low temperature, showsthe potential for the bioremediation of nitrobenzene contaminated environment.
从一条受硝基苯污染河流的沉积物中分离出七株能够在低温下降解硝基苯的细菌菌株。其中一株菌株NB1，在2.5摄氏度至35摄氏度的温度范围内能够完全矿化20毫克/升的硝基苯，最适温度为25摄氏度。在5摄氏度时，该菌株能够在pH值为6 - 9的条件下生长并降解20毫克/升的硝基苯。只要硝基苯浓度不高于100毫克/升，该菌株就能成功降解。根据其形态、生化特性及16S rDNA序列分析，该菌株被鉴定为恶臭假单胞菌。该菌株在不同温度下，尤其是在低温下的生长及硝基苯降解特性，显示出其在生物修复硝基苯污染环境方面的潜力。
