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Unbiased amplification of a highly complex mixture of DNA fragments by 'lone linker'-tagged PCR.

作者信息

Ko M S, Ko S B, Takahashi N, Nishiguchi K, Abe K

机构信息

Furusawa MorphoGene Project, Research Development Corporation of Japan, Tsukuba.

出版信息

Nucleic Acids Res. 1990 Jul 25;18(14):4293-4. doi: 10.1093/nar/18.14.4293.

DOI:10.1093/nar/18.14.4293
PMID:2377489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331231/
Abstract
摘要

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本文引用的文献

1
Preparation of cDNA and the generation of cDNA libraries: overview.互补DNA(cDNA)的制备及cDNA文库的构建:概述
Methods Enzymol. 1987;152:307-16. doi: 10.1016/0076-6879(87)52035-3.
2
Whole genome PCR: application to the identification of sequences bound by gene regulatory proteins.全基因组PCR:在鉴定基因调控蛋白结合序列中的应用。
Nucleic Acids Res. 1989 May 25;17(10):3645-53. doi: 10.1093/nar/17.10.3645.
3
A novel cDNA/PCR strategy for efficient cloning of small amounts of undefined RNA.
一种分析专性细胞内病原体转录组的创新方法:选择性捕获绵羊泰勒虫转录序列。
BMC Mol Biol. 2009 Dec 24;10:111. doi: 10.1186/1471-2199-10-111.
4
An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis.一种用于高效高密度寡核苷酸微阵列分析的改进型单细胞cDNA扩增方法。
Nucleic Acids Res. 2006 Mar 17;34(5):e42. doi: 10.1093/nar/gkl050. Print 2006.
5
Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences.通过转录序列的选择性捕获揭示巨噬细胞内伤寒沙门氏菌血清型伤寒杆菌的转录组。
Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1906-11. doi: 10.1073/pnas.0509183103. Epub 2006 Jan 27.
6
Global analysis of Helicobacter pylori gene expression in human gastric mucosa.人胃黏膜中幽门螺杆菌基因表达的全局分析。
Gastroenterology. 2002 Nov;123(5):1637-48. doi: 10.1053/gast.2002.36589.
7
Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.通过通用PCR扩增方法从亚微克量的总RNA构建富含长转录本的cDNA文库。
Genome Res. 2001 Sep;11(9):1553-8. doi: 10.1101/gr.185501.
8
Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS).通过转录序列选择性捕获技术(SCOTS)鉴定人巨噬细胞吞噬作用下合成的结核分枝杆菌RNA。
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11554-9. doi: 10.1073/pnas.96.20.11554.
9
A systematic molecular genetic approach to study mammalian germline development.一种用于研究哺乳动物种系发育的系统分子遗传学方法。
Int J Dev Biol. 1998;42(7):1051-65.
10
The rat gene homologous to the human gene 9-27 is involved in the development of the mammary gland.与人类基因9 - 27同源的大鼠基因参与乳腺的发育。
Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1079-84. doi: 10.1073/pnas.95.3.1079.
Gene. 1989 Sep 30;81(2):295-306. doi: 10.1016/0378-1119(89)90190-x.
4
The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites.染色体小鼠β-珠蛋白主要基因的序列:帽化、剪接和聚腺苷酸化位点的同源性
Cell. 1978 Dec;15(4):1125-32. doi: 10.1016/0092-8674(78)90040-5.