通过“单链接头”标记的PCR对高度复杂的DNA片段混合物进行无偏差扩增。 - Suppr

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超能文献

Unbiased amplification of a highly complex mixture of DNA fragments by 'lone linker'-tagged PCR.

作者信息

Ko M S, Ko S B, Takahashi N, Nishiguchi K, Abe K

机构信息

Furusawa MorphoGene Project, Research Development Corporation of Japan, Tsukuba.

出版信息

Nucleic Acids Res. 1990 Jul 25;18(14):4293-4. doi: 10.1093/nar/18.14.4293.

DOI:10.1093/nar/18.14.4293

PMID:2377489

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331231/

Abstract

摘要

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Unbiased amplification of a highly complex mixture of DNA fragments by 'lone linker'-tagged PCR.通过“单链接头”标记的PCR对高度复杂的DNA片段混合物进行无偏差扩增。

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本文引用的文献

Preparation of cDNA and the generation of cDNA libraries: overview.互补DNA（cDNA）的制备及cDNA文库的构建：概述

Methods Enzymol. 1987;152:307-16. doi: 10.1016/0076-6879(87)52035-3.

Whole genome PCR: application to the identification of sequences bound by gene regulatory proteins.全基因组PCR：在鉴定基因调控蛋白结合序列中的应用。

Nucleic Acids Res. 1989 May 25;17(10):3645-53. doi: 10.1093/nar/17.10.3645.

A novel cDNA/PCR strategy for efficient cloning of small amounts of undefined RNA.

Gene. 1989 Sep 30;81(2):295-306. doi: 10.1016/0378-1119(89)90190-x.

The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites.染色体小鼠β-珠蛋白主要基因的序列：帽化、剪接和聚腺苷酸化位点的同源性

Cell. 1978 Dec;15(4):1125-32. doi: 10.1016/0092-8674(78)90040-5.

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