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Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes.对帽式捕获法筛选的cDNA进行标准化和扣除，以制备全长cDNA文库，用于快速发现新基因。
Genome Res. 2000 Oct;10(10):1617-30. doi: 10.1101/gr.145100.
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Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray.使用15,000个小鼠发育cDNA微阵列对妊娠中期胎盘和胚胎进行全基因组表达谱分析。
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):9127-32. doi: 10.1073/pnas.97.16.9127.
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Statistical analysis of the 5' untranslated region of human mRNA using "Oligo-Capped" cDNA libraries.使用“寡聚帽”cDNA文库对人类mRNA的5'非翻译区进行统计分析。
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Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse development.大规模cDNA分析揭示了小鼠植入前发育过程中的阶段性基因表达模式。
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The mammalian gene collection.哺乳动物基因文库
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An improved method for construction of directionally cloned cDNA libraries from microdissected cells.一种从显微切割细胞构建定向克隆cDNA文库的改进方法。
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Homing endonucleases: keeping the house in order.归巢内切酶：维持秩序
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通过通用PCR扩增方法从亚微克量的总RNA构建富含长转录本的cDNA文库。

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.

作者信息

Piao Y, Ko N T, Lim M K, Ko M S

机构信息

Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA.

出版信息

Genome Res. 2001 Sep;11(9):1553-8. doi: 10.1101/gr.185501.

DOI:10.1101/gr.185501
PMID:11544199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311119/
Abstract

Here we report a novel design of linker primer that allows one to differentially amplify long tracts (average 3.0 kb with size ranges of 1-7 kb) or short DNAs (average 1.5 kb with size ranges of 0.5-3 kb) from a complex mixture. The method allows one to generate cDNA libraries enriched for long transcripts without size selection of insert DNAs. One representative library from newborn kidney includes 70% of clones bearing ATG start codons. A comparable library has been generated from 20 mouse blastocysts, containing only approximately 40 ng of total RNA. This universal PCR amplification scheme can provide a route to isolate very large cDNAs, even if they are expressed at very low levels.

摘要

在此，我们报告了一种新型接头引物设计，该设计可使人们从复杂混合物中差异扩增长片段（平均3.0 kb，大小范围为1 - 7 kb）或短DNA（平均1.5 kb，大小范围为0.5 - 3 kb）。该方法使人们能够生成富含长转录本的cDNA文库，而无需对插入DNA进行大小选择。一个来自新生小鼠肾脏的代表性文库包含70%带有ATG起始密码子的克隆。已从20个小鼠囊胚中生成了一个类似的文库，其总RNA仅约40 ng。这种通用的PCR扩增方案可为分离非常大的cDNA提供一条途径，即使它们的表达水平非常低。