Wei Qiang-wei, Dong Yan-mei, Chen Xiao-feng, Li Yu-li, Miao Guo-hou
Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2013 Jun 18;45(3):484-8.
To investigate the proliferation and differentiation of the human dental pulp cells (hDPCs) on the bioactive scaffolds.
Primary HDPCs were harvested from impacted third molars of healthy adult individuals (18-25 years of age) by enzyme digestion, expanded and cultured. The cells used for this investigation were the 4 th passage. Immunohistochemical methods were used to verify that the cells were dental pulp cells. The expression of stromal precursor antigen-1 (STRO-1) was determined by flow cytometry. Three different types of scaffolds were used: collagen (COL), collagen / bioglass (COL-BG) and collagen / bioglass / polycaprolactone (COL-BG-PCL). Cell proliferation on the scaffolds was determined using a MTT assay at hour 6, on days 1, 3, 5, 7, 14 and 21. On day 14, the scaffolds were stained with the alkaline phosphatase (ALP) staining kit.
The tested cells had STRO-1 positive cells. The proliferation of HDPCs was significantly higher on the COL-BG scaffold and COL-BG-PCL scaffold as compared with COL scaffold (P<0.05). Especially on days 14 and 21, the optical density value of bioglass composite scaffold were 5 times that of the COL scaffold. The ALP positive staining area was observed more extensively on the COL-BG scaffold and COL-BG-PCL scaffold than on the COL scaffold.
As comparison with the COL scaffold, HDPCs' proliferation and differentiation present more activity on the COL-BG and COL-BG-PCL scaffolds.
