Mi Shan-shan, Dong Yan-mei, Gao Xue-jun
Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2012 Feb 18;44(1):39-42.
To evaluate the effects of ionic-dissolution of sol-gel bioactive glasses (BG) on proliferation and alkaline phosphatase (ALP) activity of human dental pulp cells (HDPCs) .
The primary HDPCs were isolated from intact premolar and third molars and were cultured in DMEM. Then the 4 th generation of HDPCs were cultured in DMEM, which contained 1 g/L of one of 58S, Nano-58S, and 45S5 ionic-dissolution products. Meanwhile HDPCs were cultured in DMEM without BG as control group. Proliferation of the cells was evaluated by MTT assay on days 1,3,5 and 7. ALP activity was measured on day 14 using ALP Assay Kit.
Compared with the control group, HDPCs' proliferation in the Nano-58S group increased significantly. HDPCs cultured in Nano-58S and the 58S groups showed higher ALP activity (P<0.01); but in 45S5 group showed an inhibition on the HDPCs' proliferation (P<0.01) and lower ALP activity (P<0.05).
Present work has shown that Nano-58S and 58S sol-gel BG can promote the proliferation and ALP activity of HDPCs.
评估溶胶-凝胶生物活性玻璃（BG）的离子溶解对人牙髓细胞（HDPCs）增殖和碱性磷酸酶（ALP）活性的影响。
从完整的前磨牙和第三磨牙中分离出原代HDPCs，并在DMEM中培养。然后将第4代HDPCs培养于含有58S、纳米58S和45S5这5种离子溶解产物之一（浓度为1 g/L）的DMEM中。同时，将HDPCs培养于不含BG的DMEM中作为对照组。在第1、3、5和7天通过MTT法评估细胞增殖情况。在第14天使用ALP检测试剂盒测量ALP活性。
与对照组相比，纳米58S组中HDPCs的增殖显著增加。在纳米58S组和58S组中培养的HDPCs显示出较高的ALP活性（P<0.01）；但在45S5组中对HDPCs的增殖有抑制作用（P<0.01）且ALP活性较低（P<0.05）。
目前的研究表明，纳米58S和58S溶胶-凝胶BG可促进HDPCs的增殖和ALP活性。
