Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Avenida Colombo 5790, Bloco H67 (11), Jardim Universitário, CEP: 87020-900, Maringá, Paraná, Brazil.
J Sci Food Agric. 2014 Feb;94(3):453-8. doi: 10.1002/jsfa.6268. Epub 2013 Jul 16.
Cassava (Manihot esculenta Crantz), a plant used as food and an ingredient in industry, contains cyanogenic glycosides. The cassava root contains wastewater, popularly known as manipueira, which is a toxic substance. Its ingestion by animals causes poisoning although they react positively to treatment with sodium thiosulfate. The present research evaluates the cytotoxicity and the mutagenicity of liquid waste produced in the process of industrialization of the bitter cassava, olho-junto variety. The liquid wastes are characterized as press water, which is obtained when the cassava roots are pressed; pond water, which is press water stored in impounded ponds; and a solution of sodium thiosulfate, pure and with other waste.
The system tests comprised root meristematic cells of Allium cepa L. and bone marrow cells of Rattus norvegicus. Treatment with saline solution was cytotoxic for Allium cepa L. and significantly reduced cell division rate. Although no treatment was cytotoxic in any of the tests with rats, the thiosulfate solution was clastogenic for the chromosomal aberrations test.
Since it is harmful to the genetic material submitted within the conditions of current research, sodium thiosulfate should only be used in emergency conditions in which the benefits exceed the risks.
木薯（Manihot esculenta Crantz）是一种用作食物和工业原料的植物，含有氰苷糖苷。木薯根含有废水，俗称 manipueira，是一种有毒物质。动物摄入后会中毒，但用硫代硫酸钠处理会产生积极反应。本研究评估了工业加工苦味木薯（olho-junto 品种）过程中产生的液态废物的细胞毒性和致突变性。液态废物的特点是压榨水，即压榨木薯根时获得的水；池塘水，即储存在池塘中的压榨水；以及纯硫代硫酸钠溶液和其他废物的溶液。
系统测试包括洋葱根尖细胞和大鼠骨髓细胞。生理盐水处理对洋葱根尖细胞有细胞毒性，显著降低了细胞分裂率。虽然在任何大鼠试验中都没有处理是细胞毒性的，但硫代硫酸盐溶液对染色体畸变试验具有致裂作用。
由于它对当前研究条件下提交的遗传物质有害，硫代硫酸钠仅应在效益超过风险的紧急情况下使用。
