Ali Syeda Roohina, Haque Sayedul, Mudassar -, Versiani Muhammad Ali, Faizi Shaheen, Farooq Ahsana Dar
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan.
Pak J Pharm Sci. 2017 Mar;30(2):521-529.
Cuscuta reflexa (Convolvulaceae), is commonly known as amarbel or akashbel. In Bangladesh and Nepal some of the tribes use C. reflexa against edema, body ache, cancer, skin infections and liver disorders. Despite its traditional uses there is no information regarding genotoxic effects of either the plant extract or its pure compounds. Methanolic extract of C. reflexa (MECR) and pure compounds derived from it namely, odoroside H, neritaloside, and strospeside, were evaluated in Allium cepa L. and A. sativum L. for their effects on root growth, root apical meristem mitotic index (MI) , and chromosomal aberrations (CAs). In this study, we adopted a new method of calculating percent change in root length. MECR caused a concentration- and time- dependent inhibition in root length at 100 - 10000μg/ml in A. cepa root. It was accompanied by a subsequent decline in MI which is an indicative of its cytotoxic effect. On the contrary, at low concentrations a significant rise in root length was noticeable. In A. sativum, MECR also reduced the root length having IC values ~8 x and 4.3 x lower than A. cepa. A variety of CAs were evident in both Allium systems after treatment with MECR, odoroside H and neritaloside. Thus in MECR, cardenolides glycosides, i.e. odoroside H and neritaloside could be accountable for its genotoxicity.
菟丝子（旋花科），通常被称为列当或阿卡萨贝。在孟加拉国和尼泊尔，一些部落使用菟丝子来治疗水肿、身体疼痛、癌症、皮肤感染和肝脏疾病。尽管有这些传统用途，但关于该植物提取物或其纯化合物的遗传毒性作用尚无相关信息。对菟丝子的甲醇提取物（MECR）及其衍生的纯化合物，即奥多罗苷H、奈里塔罗苷和斯特罗斯苷，在洋葱和大蒜中进行了评估，以研究它们对根生长、根尖分生组织有丝分裂指数（MI）和染色体畸变（CAs）的影响。在本研究中，我们采用了一种计算根长度百分比变化的新方法。在洋葱根中，MECR在100 - 10000μg/ml浓度下对根长度产生浓度和时间依赖性抑制。同时伴随着MI的随后下降，这表明其具有细胞毒性作用。相反，在低浓度下，根长度有显著增加。在大蒜中，MECR也降低了根长度，其IC值比洋葱低约8倍和4.3倍。用MECR、奥多罗苷H和奈里塔罗苷处理后，两种葱属植物系统中都出现了多种染色体畸变。因此，在MECR中，强心苷糖苷，即奥多罗苷H和奈里塔罗苷可能是其遗传毒性的原因。
