Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
Biomaterials. 2013 Sep;34(28):6967-75. doi: 10.1016/j.biomaterials.2013.05.059. Epub 2013 Jun 15.
As an excellent photothermal agent candidate, gold nanoshells have attracted a great deal of attention, but the influences of PEGylation on their biological effects and light heat conversion efficiency remain unclear. Here we investigate the influences of PEGylation density on the gold nanoshells on silica nanorattles (GSNPs) to their biological effects, including their cellular uptake, "corona" of biological macromolecules they are covered with, in vivo biodistribution and toxicities, and their in vitro and in vivo light heat conversion efficiency. The results suggest PEGylation obviously impacts the uptake patterns of GSNPs. Less-density PEGylated GSNPs show enhanced cellular uptake caused by the high dose exposure on cell surface due to their rapid aggregation. High-density PEGylated GSNPs show advantages in less toxicity for suppression of aggregation of GSNPs, avoidance of RES, good enhanced permeability and retention (EPR) effect of cancerous tumors, especially the enhanced light heat conversion efficiency in vivo. Less or insufficient PEGylation may induce in vivo toxicity. This study highlights the need to study the effect of PEGylation for near infrared (NIR) light absorbing nanoparticles to predict the effects and safety of nanotherapeutics.
作为一种优秀的光热试剂候选物，金纳米壳已经引起了广泛关注，但聚乙二醇（PEG）化对其生物效应和光热转换效率的影响尚不清楚。在这里，我们研究了聚乙二醇（PEG）化密度对金纳米壳-二氧化硅纳米花（GSNPs）的影响，以及它们对生物效应的影响，包括它们的细胞摄取、它们所覆盖的生物大分子的“冠”、体内分布和毒性，以及它们在体外和体内的光热转换效率。结果表明，聚乙二醇（PEG）化明显影响 GSNPs 的摄取模式。由于细胞表面高剂量暴露导致的快速聚集，低密度聚乙二醇（PEG）化 GSNPs 表现出增强的细胞摄取。高密度聚乙二醇（PEG）化 GSNPs 具有较低的毒性优势，可抑制 GSNPs 的聚集，避免 RES，对癌细胞具有良好的增强渗透性和保留（EPR）效应，特别是在体内增强光热转换效率。过少或不足的聚乙二醇（PEG）化可能会导致体内毒性。这项研究强调了需要研究近红外（NIR）光吸收纳米颗粒的聚乙二醇（PEG）化效应，以预测纳米治疗剂的效果和安全性。
