CD133+ 和 CXCR4+ 结肠癌细胞作为淋巴结转移的标志物。

CD 133+ and CXCR4+ colon cancer cells as a marker for lymph node metastasis.

机构信息

Department of Colon and Rectal Surgery, Ochsner Clinic Foundation, New Orleans, Louisiana.

出版信息

J Surg Res. 2013 Nov;185(1):113-8. doi: 10.1016/j.jss.2013.05.049. Epub 2013 Jun 5.

DOI:10.1016/j.jss.2013.05.049

PMID:23777983

Abstract

INTRODUCTION

Colorectal cancer (CRC) stem cells or tumor-initiating cells (Co-TIC) are implicated in both cancer recurrence and extranodal metastasis. CD133 and CXCR4 are specific cell surface markers that are indicators of Co-TIC. The presence of lymph node (LN) metastases is one of the strongest negative prognostic factors for CRC patients. We examined the relationship between the Co-TIC markers CD133 and CXCR4 and LN involvement in CRC.

METHODS

CRC cells were isolated via enzymatic digestion. CD133(+), CXCR4(+), and double-positive CRC cells were detected by fluorescence-activated cell sorting analysis. The percentages of CD133(+), CXCR4(+), and double-positive cells were identified and correlated to the number and percentage of positive LN on staging.

RESULTS

Twenty-seven samples underwent fluorescence-activated cell sorting analysis. The mean percentage of CD133(+) cells was 3.94% (range 0.15%-19.06%). The mean percentage of CXCR4(+) cells was 6.15% (range 0%-27.11%). The mean percentage of CD133(+)CXCR4(+) cells was 0.45% (range 0%-2.08%). Thirteen patients had LN metastasis: 8 N1 disease and 5 N2 disease. The correlation coefficients between the percentage of Co-TIC marker-positive cells and percentage of positive LN were r = 0.58 (P = 0.0016) for CD133(+) cells, r = 0.36 (P = 0.5868) for CXCR4(+) cells, and r = 0.56 (P = 0.0022) for double-positive cells.

DISCUSSION

Our results show CD133(+) and CD133(+)CXCR4(+) cancer cells correlate with the presence of LN metastasis in CRC. Further studies will examine whether these markers can give consistent prognostic information and may help to develop novel diagnostic and therapeutic options.

摘要

简介

结直肠癌（CRC）干细胞或肿瘤起始细胞（Co-TIC）被认为与癌症复发和结外转移有关。CD133 和 CXCR4 是 Co-TIC 的特定细胞表面标志物。淋巴结（LN）转移的存在是 CRC 患者最强的负预后因素之一。我们研究了 Co-TIC 标志物 CD133 和 CXCR4 与 CRC 中 LN 受累之间的关系。

方法

通过酶消化分离 CRC 细胞。通过荧光激活细胞分选分析检测 CD133(+)、CXCR4(+)和双阳性 CRC 细胞。鉴定 CD133(+)、CXCR4(+)和双阳性细胞的百分比，并将其与分期时 LN 阳性的数量和百分比相关联。

结果

27 个样本进行了荧光激活细胞分选分析。CD133(+)细胞的平均百分比为 3.94%（范围 0.15%-19.06%）。CXCR4(+)细胞的平均百分比为 6.15%（范围 0%-27.11%）。CD133(+)CXCR4(+)细胞的平均百分比为 0.45%（范围 0%-2.08%）。13 例患者有 LN 转移：8 例 N1 疾病，5 例 N2 疾病。Co-TIC 标志物阳性细胞百分比与 LN 阳性百分比之间的相关系数为 CD133(+)细胞 r = 0.58（P = 0.0016），CXCR4(+)细胞 r = 0.36（P = 0.5868），双阳性细胞 r = 0.56（P = 0.0022）。