Anton H J, Oberemm A
Zoological Institute, University of Cologne, Federal Republic of Germany.
Stain Technol. 1990;65(3):119-23. doi: 10.3109/10520299009139924.
A method has been developed to obtain horseradish peroxidase-treated serial sections containing spinal cord as well as bilateral ventral and dorsal roots, dorsal root ganglia and spinal nerves. Young postmetamorphic newts (Triturus alpestris) served as experimental animals. After cryotome cross sectioning the forelimb region of the trunk, slices 80 microns in thickness were mounted serially with up to 15 sections per slide. This facilitated subsequent staining manipulations and made partial loss of sections less likely.
已经开发出一种方法来获取经辣根过氧化物酶处理的连续切片，这些切片包含脊髓以及双侧腹根和背根、背根神经节和脊神经。幼年变态后的蝾螈（高山蝾螈）用作实验动物。在对躯干的前肢区域进行冷冻切片横切后，将厚度为80微米的切片连续安装，每张载玻片最多可放置15个切片。这便于后续的染色操作，并降低了切片部分丢失的可能性。
