Leu Jyh-Gang, Lin Chin-Yao, Jian Jhin-Hao, Shih Chin-Yu, Liang Yao-Jen
Fu-Jen Catholic University, School of Medicine, Xinzhuang District, New Taipei City, Taiwan.
An Acad Bras Cienc. 2013 Apr-Jun;85(2):745-52. doi: 10.1590/S0001-37652013005000023. Epub 2013 Jun 17.
The anti-oxidant effects of epigallocatechin gallate (EGCG) and alpha lipoic acid (ALA) have been demonstrated in previous studies. The kidney protection effects of EGCG and ALA in patients with kidney injury are still under investigation. The purpose of this study is to investigate the anti-inflammatory and anti-oxidant effects of EGCG and ALA on high glucose-induced human kidney cell damage. EGCG inhibited high glucose(HG)-induced TNF-α and IL-6 production in human embryonic kidney (HEK) cells. Both EGCG and ALA decreased HG-induced receptor of advanced glycation end products (RAGE) mRNA and protein expressions in HEK cells. EGCG and ALA also recovered HG-inhibited superoxide dismutase production and decreased ROS expressions in HEK cells. The synergism of EGCG and ALA was also studied. The effect of EGCG combined with ALA is greater than the effect of EGCG alone in all anti-inflammation and anti-oxidant experiments. Our studies provide a potential therapeutic application of EGCG and ALA in preventing progression of diabetic nephropathy.
表没食子儿茶素没食子酸酯（EGCG）和α硫辛酸（ALA）的抗氧化作用已在先前的研究中得到证实。EGCG和ALA对肾损伤患者的肾脏保护作用仍在研究中。本研究的目的是探讨EGCG和ALA对高糖诱导的人肾细胞损伤的抗炎和抗氧化作用。EGCG抑制高糖（HG）诱导的人胚肾（HEK）细胞中肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的产生。EGCG和ALA均降低了HG诱导的HEK细胞中晚期糖基化终产物受体（RAGE）的mRNA和蛋白表达。EGCG和ALA还恢复了HG抑制的超氧化物歧化酶的产生，并降低了HEK细胞中活性氧（ROS）的表达。还研究了EGCG和ALA的协同作用。在所有抗炎和抗氧化实验中，EGCG与ALA联合使用的效果大于单独使用EGCG的效果。我们的研究为EGCG和ALA在预防糖尿病肾病进展方面提供了潜在的治疗应用。
