Department of Chemistry, Faculty of Science, Selcuk University, Konya, 42075, Turkey.
Appl Biochem Biotechnol. 2013 Aug;170(8):1871-84. doi: 10.1007/s12010-013-0308-x. Epub 2013 Jun 19.
The article describes preparation of new calixarene biopolymers consisting of the immobilization of convenience calixarene derivative onto cellulose and chitosan biopolymers, and the encapsulation of these calixarene biopolymers with Candida rugosa lipase within a chemical inert sol-gel supported by polycondensation with tetraethoxysilane and octyltriethoxysilane. The catalytic properties of immobilized lipase were evaluated into model reactions employing the hydrolysis of p-nitrophenylpalmitate and the enantioselective hydrolysis of naproxen methyl esters from racemic prodrugs in aqueous buffer solution/isooctane reaction system. The resolution studies using sol-gel support have observed more improvement in the enantioselectivity of naproxen E = 300 with Cel-Calix-E than with encapsulated lipase without calixarene-based materials. Furthermore, the encapsulated lipase (Cel-Calix-E) was still retained about 39 % of their conversion ratios after the fifth reuse in the enantioselective reaction.
本文描述了新型杯芳烃生物聚合物的制备，该聚合物由方便的杯芳烃衍生物固定在纤维素和壳聚糖生物聚合物上，并通过与四乙氧基硅烷和辛基三乙氧基硅烷缩聚形成的化学惰性溶胶-凝胶内包封这些杯芳烃生物聚合物中的 Candida rugosa 脂肪酶。在水缓冲溶液/异辛烷反应体系中，通过对 p-硝基苯棕榈酸酯的水解和外消旋前药中萘普生甲酯的对映选择性水解的模型反应评价了固定化脂肪酶的催化性能。使用溶胶-凝胶载体的拆分研究观察到 Cel-Calix-E 中的萘普生 E = 300 的对映选择性比没有杯芳烃基材料的包封脂肪酶有了更多的提高。此外，在对映选择性反应中，包封的脂肪酶（Cel-Calix-E）在第五次重复使用后仍保留约 39%的转化率。
