Pelegrini Camila Bonvicino, Maia Luciana Prado, de Souza Sérgio Luís Scombatti, Taba Mário, Palioto Daniela Bazan
Department of Traumatology and Bucomaxillofacial Surgery and Periodontology, Ribeirão Preto Dental School, USP - University of São Paulo, Ribeirão Preto, SP, Brazil.
Braz Dent J. 2013;24(2):128-35. doi: 10.1590/0103-6440201302144.
As dogs are good models for in vivo studies, it is interesting to evaluate the behavior of canine gingival fibroblasts (CGF) in vitro, so that these cells could be seeded on a matrix and later studied in vivo. The aim of this study was to perform a morphological, functional and biochemical analysis of CGF, comparing it with human gingival fibroblasts (HGF), as well as to evaluate the change of their characteristics over several passages. Using gingival fibroblasts from 3 dogs and 3 humans in the subculture (Sub), first (P1), third (P3), fifth (P5) and seventh (P7) passages, the following parameters were assessed: cell morphology, spreading, adhesion, viability and total protein content. The results showed no major differences between the passages in terms of morphology and spreading, and a tendency of greater adhesion and viability for HGF when compared with CGF. The total protein content was significantly higher for HGF. HGF exhibited greater functional and biochemical activity in vitro compared to CGF. Higher numbers at Sub were observed for both CGF and HGF in all evaluated parameters. The differences do not prevent the use of CGF for tissue engineering, but its use seems to be more appropriate in the subculture or first passage.
由于狗是体内研究的良好模型，因此在体外评估犬牙龈成纤维细胞（CGF）的行为很有趣，这样这些细胞就可以接种在基质上，随后在体内进行研究。本研究的目的是对CGF进行形态学、功能和生化分析，并将其与人类牙龈成纤维细胞（HGF）进行比较，同时评估它们在传代过程中的特性变化。使用来自3只狗和3个人的牙龈成纤维细胞进行传代培养（Sub）、第一代（P1）、第三代（P3）、第五代（P5）和第七代（P7），评估以下参数：细胞形态、铺展、黏附、活力和总蛋白含量。结果显示，在形态和铺展方面各代之间没有显著差异，与CGF相比，HGF有更强的黏附力和活力倾向。HGF的总蛋白含量显著更高。与CGF相比，HGF在体外表现出更大的功能和生化活性。在所有评估参数中，Sub代的CGF和HGF数量都更高。这些差异并不妨碍将CGF用于组织工程，但在传代培养或第一代使用它似乎更合适。
