Nagy Krisztina, Láng Orsolya, Láng Júlia, Perczel-Kovách Katalin, Gyulai-Gaál Szabolcs, Kádár Kristóf, Kőhidai László, Varga Gábor
Department of Oral Biology, Semmelweis University, Budapest, Hungary.
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
Interv Med Appl Sci. 2018 Sep;10(3):162-170. doi: 10.1556/1646.10.2018.21.
Periodontal ligament stem cells (PDLSCs) possess extensive regeneration potential. However, their therapeutic application demands a scaffold with appropriate properties. HydroMatrix (HydM) is a novel injectable peptide nanofiber hydrogel developed recently for cell culture. Our aim was to test whether HydM would be a suitable scaffold for proliferation and osteogenic differentiation of PDLSCs. PDLSCs were seeded on non-coated or HydM-coated surfaces. Both real-time impedance analysis and cell viability assay documented cell growth on HydM. PDLSCs showed healthy, fibroblast-like morphology on the hydrogel. After a 3-week-long culture in osteogenic medium, mineralization was much more intense in HydM cultures compared to control. Alkaline phosphatase activity of the cells grown on the gels reached the non-coated control levels. Our data provided evidence that PDLSCs can adhere, survive, migrate, and proliferate on HydM and this gel also supports their osteogenic differentiation. We first applied impedimetry for dental stem cells cultured on a scaffold. HydM is ideal for studies of PDLSCs. It may also serve not only as a reference material but also in the future as a promising biocompatible scaffold for preclinical studies.
牙周膜干细胞（PDLSCs）具有广泛的再生潜力。然而，它们的治疗应用需要具有适当特性的支架。水凝胶基质（HydM）是最近开发的一种用于细胞培养的新型可注射肽纳米纤维水凝胶。我们的目的是测试HydM是否会是一种适合PDLSCs增殖和成骨分化的支架。将PDLSCs接种在未包被或HydM包被的表面上。实时阻抗分析和细胞活力测定均记录了HydM上的细胞生长情况。PDLSCs在水凝胶上呈现出健康的、成纤维细胞样形态。在成骨培养基中培养3周后，与对照组相比，HydM培养物中的矿化更为强烈。在凝胶上生长的细胞的碱性磷酸酶活性达到了未包被对照组的水平。我们的数据表明，PDLSCs可以在HydM上黏附、存活、迁移和增殖，并且这种凝胶还支持它们的成骨分化。我们首次将阻抗分析法应用于在支架上培养的牙干细胞。HydM是研究PDLSCs的理想材料。它不仅可以作为参考材料，而且在未来还可能成为一种有前景的用于临床前研究的生物相容性支架。
