Tvedten Harold W, Ljusner Jessika, Lilliehöök Inger E
Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Sciences, Uppsala, Sweden.
J Vet Diagn Invest. 2013 Jul;25(4):493-7. doi: 10.1177/1040638713493419. Epub 2013 Jun 18.
A manual method (Thrombo-TIC; Bioanalytic GmbH, Umkirch/Freiburg, Germany) was advertised to disaggregate platelet clumps and to make human platelets spherical to improve platelet enumeration. The current study's hypothesis was that this method would perform better than current methods for feline blood anticoagulated with ethylenediamine tetra-acetic acid (EDTA), which often contains platelet aggregates. Platelet concentrations (PLTs) were determined in 21 feline blood samples by 3 methods. Thrombo-TIC was compared to the manual method (Leucoplate; Sobioda, Montbonnot-Saint-Martin, France) currently used in the authors' laboratory along with an ADVIA 2120 (Siemens AG, Eschborn, Germany) optical platelet concentration. Feline blood samples often contained platelet aggregates. ADVIA flagged for platelet aggregates in 11 of the 21 feline blood samples, and examination of blood smear revealed platelet aggregates in 14 of the 21 samples. The hemocytometers displayed more platelet aggregates with the Thrombo-TIC method than with Leucoplate. The method giving the greatest PLT was considered most accurate. The Leucoplate median PLT (238 × 10(9)/1) was greater than Thrombo-TIC (202 × 10(9)/1) or ADVIA (157 × 10(9)/1). Intra-assay precision was determined for the 3 methods using the 21 feline blood samples. Median Thrombo-TIC and Leucoplate precision (7.4% and 7.3% coefficient of variation [CV], respectively) were similar and not much worse than ADVIA (5.9% CV). The Thrombo-TIC method did not appear to perform better than the current manual method (Leucoplate). Leucoplate appeared least affected by platelet aggregation in feline blood. The ADVIA automated PLT appeared to be most negatively affected by platelet aggregation. The Thrombo-TIC method did not appear to prevent platelet aggregation in feline EDTA blood samples and, thus, is not recommended for such use.
有一种手动方法（Thrombo-TIC；德国乌姆基希/弗赖堡的Bioanalytic GmbH公司）宣称可使血小板凝块解聚，并使人类血小板呈球形，以改善血小板计数。本研究的假设是，对于用乙二胺四乙酸（EDTA）抗凝的猫血（这种血通常含有血小板聚集体），该方法比现有方法表现更好。通过3种方法测定了21份猫血样本中的血小板浓度（PLTs）。将Thrombo-TIC与作者实验室目前使用的手动方法（Leucoplate；法国蒙博诺-圣马丁的Sobioda公司）以及ADVIA 2120（德国埃施博恩的西门子公司）光学血小板浓度测定法进行了比较。猫血样本中常常含有血小板聚集体。在21份猫血样本中，ADVIA标记出11份存在血小板聚集体，对血涂片的检查显示2
