脂多糖 O 抗原多糖在无血清条件下诱导 RAW264.7 细胞产生一氧化氮。

Induction of nitric oxide production in RAW264.7 cells under serum-free conditions by O-antigen polysaccharide of lipopolysaccharide.

机构信息

Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kagawa, Japan.

出版信息

Anticancer Res. 2013 Jul;33(7):2875-9.

Abstract

BACKGROUND

Lipopolysaccharide derived from Pantoea agglomerans (LPSp) mainly consists of two aggregates, the high-molecular aggregate (HMM-LPSp) and the low-molecular aggregate (LMM-LPSp). The structural differences between HMM-LPSp and LMM-LPSp seem to depend on the length of the O-antigen polysaccharide because the lipid A regions of the two fractions are quite similar. In this study, we examined the biological activity of LPSp focusing on the O-antigen polysaccharide using HMM-LPSp and LMM-LPSp under serum-free conditions.

MATERIALS AND METHODS

The binding of LPSp to RAW264.7 cells under serum-free conditions was analyzed by flow cytometry using LPSp-conjugated fluorescein isothiocyanate (FITC-LPSp). The biological activities of HMM-LPSp and LMM-LPSp under serum-free conditions were evaluated by the nitric oxide production.

RESULTS

FITC-LPSp showed higher fluorescence intensity under serum-free than serum-containing conditions. HMM-LPSp induced higher nitric oxide production than LMM-LPSp under serum-free conditions.

CONCLUSION

The present study indicates that the reactivity of LPSp is affected by the O-antigen polysaccharide under serum-free conditions.

摘要

背景

聚生泛菌来源的脂多糖（LPSp）主要由两个聚集物组成，高分子聚集物（HMM-LPSp）和低分子聚集物（LMM-LPSp）。HMM-LPSp 和 LMM-LPSp 之间的结构差异似乎取决于 O-抗原多糖的长度，因为这两个部分的脂质 A 区域非常相似。在这项研究中，我们使用 HMM-LPSp 和 LMM-LPSp 在无血清条件下，重点研究 LPSp 的 O-抗原多糖的生物活性。