Gmoshinskiĭ I V, Mazo V K, Shatskaia N G, Ushakov V V
Vopr Pitan. 1990 Mar-Apr(2):54-8.
The capacities of immunoelectrophoresis in identification and assay of antigenic protein in enzymatic hydrolysates of milk and soya have been evaluated. The hydrolysates obtained under the action of pancreatin and protosubtilin were studied in milk protein concentrates "Dialact" and casezit and soya protein isolate "Adrex". Two-dimensional immunoelectrophoresis according to Clarke and Freeman was used to analyze the antigenic component spectrum in the samples. It has been shown that beta-lactoglobulin, as compared to other milk proteins, is most resistant to pancreatin-induced proteolysis in vitro. Pancreatin-induced proteolysis has been shown to be useful for trypsin inhibitor inactivation in soya protein isolate. It was demonstrated that immunoelectrophoresis could be used for complex evaluation of protein hydrolysates intended for manufacturing specialized foodstuffs.
免疫电泳在鉴定和测定牛奶和大豆酶解产物中的抗原性蛋白质方面的能力已得到评估。研究了在胰蛋白酶和原枯草杆菌蛋白酶作用下获得的水解产物，这些水解产物来自牛奶蛋白浓缩物“Dialact”和酪蛋白以及大豆分离蛋白“Adrex”。采用克拉克和弗里曼的二维免疫电泳法分析样品中的抗原成分谱。结果表明，与其他牛奶蛋白相比，β-乳球蛋白在体外对胰蛋白酶诱导的蛋白水解作用最具抗性。已证明胰蛋白酶诱导的蛋白水解作用可用于使大豆分离蛋白中的胰蛋白酶抑制剂失活。结果表明，免疫电泳可用于对用于生产特殊食品的蛋白质水解产物进行综合评估。
