Tamae Kazuyoshi, Eto Toshiharu, Aoki Kazuhiro, Nakamaru Shingo, Koshikawa Kazunori, Sakuma Kazuhiko, Hirano Takeshi
Faculty of Education and Welfare Science, Oita University, Oita city, Oita, 870-1192, Japan.
Curr Aging Sci. 2013 Dec;6(3):258-65. doi: 10.2174/18746098112059990032.
Evidence based on epidemiologic investigations using biochemical parameter is meaningful for health promotion and administration among adolescents. We conducted Reactive Oxygen Metabolites (ROM) and Biological Antioxidant Potentials (BAP) tests, along with a questionnaire survey, for a sample of 74 high school students (16.51±0.11 years of aged mean±SE), to investigate the associations between ROM, BAP, and related factors, including BMI and blood biochemical data. Venous blood samples (approximately 7cc) were collected. At the same time, each individual's information was obtained from the questionnaire. The mental health status was investigated using the Center for Epidemiologic Study Depression scale (CES-D) included in the same questionnaire. The mean values and standard errors of all variables were calculated. In addition, the relationships between ROM and BAP with these factors were analyzed. The results revealed the preferred levels of ROM (261.95 ± 9.52 U.CARR) and, BAP (2429.89±53.39 µmol/L) and blood biochemical data. Few significant relationships between two markers and related factors were found. So, we detected a cluster with an imbalance between ROM and BAP, which means low antioxidant ability, whereas the other clusters had conditions with moderate balance or good balance between them. Moreover, we determined the Oxidative stress-Antioxidant capacity ratio (OAR), using the ROM and BAP values, in order to clarify the characteristic of the detected clusters.However, comparative analyses across the three clusters did not yield significant differences in all related factors. No correlations between ROM, BAP and related factors were indicated, although significant association between ROM and BAP was observed (R2=0.1156, R=0.340, P=0.013). The reason for these results can be explained by the influences of good health and young age. On the other hand, present study suggests that some latent problems among adolescents may be related to unhealthy conditions in the future. Also, this study indicated that ROM and BAP may be useful as markers of the oxidative stress status. After this, further investigations using biomarkers based on epidemiologic design should be conducted, to reveal the reliability of the present results.
基于使用生化参数的流行病学调查证据对于青少年的健康促进和管理具有重要意义。我们对74名高中生（平均年龄16.51±0.11岁，均值±标准误）进行了活性氧代谢产物（ROM）和生物抗氧化潜能（BAP）测试，并开展了问卷调查，以研究ROM、BAP与包括BMI和血液生化数据在内的相关因素之间的关联。采集了静脉血样本（约7cc）。同时，通过问卷获取每个人的信息。使用同一问卷中包含的流行病学研究中心抑郁量表（CES-D）调查心理健康状况。计算了所有变量的均值和标准误。此外，分析了ROM和BAP与这些因素之间的关系。结果显示了ROM（261.95±9.52 U.CARR）和BAP（2429.89±53.39 µmol/L）的理想水平以及血液生化数据。在两个标志物与相关因素之间未发现显著关系。因此，我们检测到一个ROM和BAP失衡的聚类，这意味着抗氧化能力较低，而其他聚类则处于中度平衡或良好平衡状态。此外，我们使用ROM和BAP值确定了氧化应激-抗氧化能力比值（OAR），以阐明所检测聚类的特征。然而，对三个聚类进行的比较分析在所有相关因素上均未产生显著差异。尽管观察到ROM和BAP之间存在显著关联（R2 = 0.1156，R = 0.340，P = 0.013），但未表明ROM、BAP与相关因素之间存在相关性。这些结果的原因可以通过良好健康状况和年轻年龄的影响来解释。另一方面，本研究表明青少年中的一些潜在问题可能与未来的不健康状况有关。此外，本研究表明ROM和BAP可能作为氧化应激状态的标志物有用。在此之后，应基于流行病学设计使用生物标志物进行进一步调查，以揭示本研究结果的可靠性。
