Brkljača Bottegaro Nika, Gotić Jelena, Šuran Jelena, Brozić Diana, Klobučar Karla, Bojanić Krunoslav, Vrbanac Zoran
Clinic for Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
BMC Vet Res. 2018 Jul 6;14(1):216. doi: 10.1186/s12917-018-1540-y.
Oxidative stress (OS) associated with an intense exercise may have a negative influence on equine health. The aim of this study was to determine the effects of endurance races on oxidative and antioxidative status of horses by evaluating changes in reactive oxygen metabolites (d-ROMs), malondialdehyde (MDA), biological antioxidant potential (BAP) and oxidative stress index (OSI) values. The study was carried out on 53 race starts (28 individual horses) competing at different endurance races according to distance (40 and 80 km) and difficulty (easy and demanding). Blood samples were taken before and after the race.
Compared to levels of OS serum biomarkers before the race, an increase in values of d-ROMs (P < 0.01), MDA (P < 0.01), and BAP (P < 0.001), and a decrease in OSI (P < 0.001) have been noted after the race. Contrary to other measured biomarkers, BAP did not show significant individual effects of horses. Horses competing at shorter races have shown a significant change in d-ROMs (P = 0.002), BAP (P < 0.001) and OSI (P = 0.004), whereas those competing at longer races in MDA (P = 0.002), BAP (P < 0.001) and OSI (P < 0.001) post-race values. Endurance racing induced changes in values of d-ROMs, BAP and OSI during both easy and demanding races.
Changes in all measured OS biomarkers indicate that prolonged aerobic exercise during endurance race could contribute to the imbalance between oxidants and antioxidants in horses, mainly characterised by a pronounced antioxidant response. Biological antioxidant potential was found to be the most reliable biomarker of OS in endurance horses in the present study.
与高强度运动相关的氧化应激（OS）可能会对马的健康产生负面影响。本研究的目的是通过评估活性氧代谢产物（d-ROMs）、丙二醛（MDA）、生物抗氧化潜力（BAP）和氧化应激指数（OSI）值的变化，确定耐力赛对马的氧化和抗氧化状态的影响。该研究对53场不同距离（40公里和80公里）和难度（轻松和艰苦）的耐力赛中的参赛马匹（28匹个体马）进行。在比赛前后采集血样。
与比赛前的OS血清生物标志物水平相比，赛后d-ROMs（P < 0.01）、MDA（P < 0.01）和BAP（P < 0.001）的值升高，OSI（P < 0.001）的值降低。与其他测量的生物标志物相反，BAP没有显示出马匹的显著个体效应。参加较短距离比赛的马匹在d-ROMs（P = 0.002）、BAP（P < 0.001）和OSI（P = 0.004）方面有显著变化，而参加较长距离比赛的马匹在赛后MDA（P = 0.002）、BAP（P < 0.001）和OSI（P < 0.001）值方面有变化。在轻松和艰苦的比赛中，耐力赛都会引起d-ROMs、BAP和OSI值的变化。
所有测量的OS生物标志物的变化表明，耐力赛中的长时间有氧运动可能导致马体内氧化剂和抗氧化剂之间的失衡，主要表现为明显的抗氧化反应。在本研究中，生物抗氧化潜力被发现是耐力马中OS最可靠的生物标志物。
