Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
J Agric Food Chem. 2013 Jul 3;61(26):6259-64. doi: 10.1021/jf401323s. Epub 2013 Jun 19.
An LC-MS/MS method was developed and validated for the determination of deoxynivalenol in wheat dust. Extraction was carried out with acetonitrile/water/acetic acid (79/20/1, v/v/v) followed by a hexane defatting step. Analysis was performed using a Waters Acquity UPLC system coupled to a Quattro Premier XE mass spectrometer. The method was validated according to the criteria mentioned in Commission Decision 2002/657/EC. Due to a high contamination level of wheat dust compared to wheat, limit of detection and limit of quantitation levels of 358 ng/g and 717 ng/g, respectively, were obtained. A small survey was executed on raw wheat materials and their corresponding dust samples (n = 12). The samples were analyzed according to the developed procedure. A linear correlation (R² = 0.941) was found for the deoxynivalenol concentration in dust versus the deoxynivalenol concentration in wheat. Therefore, it would be possible to estimate the cereal contamination through dust contamination.
建立并验证了一种用于检测小麦尘中脱氧雪腐镰刀菌烯醇的 LC-MS/MS 方法。样品经乙腈/水/冰醋酸（79/20/1，v/v/v）提取，再用正己烷脱脂，采用 Waters Acquity UPLC 系统与 Quattro Premier XE 质谱仪联用进行分析。该方法按照欧盟委员会 2002/657/EC 号决定中的标准进行了验证。由于与小麦相比，小麦尘的污染水平较高，因此分别得到了 358ng/g 和 717ng/g 的检测限和定量限。对 12 份原始小麦材料及其对应的粉尘样品进行了小范围调查。按照所建立的程序对这些样品进行了分析。在粉尘中的脱氧雪腐镰刀菌烯醇浓度与小麦中的脱氧雪腐镰刀菌烯醇浓度之间发现了线性相关性（R²=0.941）。因此，可以通过粉尘污染来估计谷物的污染情况。
