Teixeira Thais Fumaco, Dezen Diogenes, Cibulski Samuel Paulo, Varela Ana Paula Muterle, Sheffer Camila Mengue, Holz Carine Lidiane, Dos Santos Helton Fernandes, Franco Ana Cláudia, Roehe Paulo Michel
Virology, FEPAGRO Saúde Animal - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul, RS, 92990-000, Brazil,
Virus Genes. 2013 Oct;47(2):276-81. doi: 10.1007/s11262-013-0940-0. Epub 2013 Jun 20.
Torque teno sus virus (TTSuV) is a member of the recently created family Anelloviridae. Two distinct species of TTSuVs, 1 (TTSuV1) and 2 (TTSuV2) have been reported so far in domestic pigs and wild boars. Although TTSuVs have not been clearly linked to any specific disease of pigs, a relation between TTSuV infections and postweaning multisystemic wasting syndrome (PMWS) has been suggested. To examine further this possibility, the present study was conducted in search for TTSuV1 and TTSuV2 genomes in tissues of PMWS and non-PMWS-affected animals. PMWS diagnosis was established by clinical signs, characteristic macroscopic and histopathologic lesions and the presence of porcine circovirus type 2 DNA. Samples of five different tissues (lungs, kidneys, livers, spleens, and lymph nodes) from PMWS-affected and non-PMWS-affected pigs were examined with two specific PCR assays developed to amplify TTSuV1 and TTSuV2 genome segments. TTSuV1 DNA was detected in tissues of non-diseased animals to significantly higher levels than in tissues of PMWS-affected pigs (p ≤ 0.001). Regarding TTSuV2, viral genomes were detected in nearly all samples from both PMWS-affected (94.7 %) and non-affected pigs (100 %), with no significant differences in the frequencies of detection of TTSuV2 genomes in both groups. No significant differences were detected on the distribution of TTSuV1 and TTSuV2 in the different tissues examined (p = 0.970).
扭矩细小病毒（TTSuV）是最近新建立的圆环病毒科的一个成员。迄今为止，在家猪和野猪中已报道了两种不同的TTSuV物种，即1型（TTSuV1）和2型（TTSuV2）。尽管TTSuV与猪的任何特定疾病之间尚未明确关联，但有人提出TTSuV感染与断奶后多系统消耗综合征（PMWS）之间存在关联。为了进一步研究这种可能性，本研究对PMWS和未受PMWS影响的动物组织中的TTSuV1和TTSuV2基因组进行了检测。通过临床症状、特征性大体和组织病理学病变以及2型猪圆环病毒DNA的存在来确诊PMWS。使用两种专门开发的用于扩增TTSuV1和TTSuV2基因组片段的特异性PCR检测方法，对受PMWS影响和未受PMWS影响的猪的五种不同组织（肺、肾、肝、脾和淋巴结）样本进行了检测。在未患病动物的组织中检测到的TTSuV1 DNA水平明显高于受PMWS影响的猪的组织（p≤0.001）。关于TTSuV2，在受PMWS影响的猪（94.7%）和未受影响的猪（100%）的几乎所有样本中都检测到了病毒基因组，两组中TTSuV2基因组的检测频率没有显著差异。在所检测的不同组织中，TTSuV1和TTSuV2的分布没有显著差异（p = 0.970）。
