Manor E, Sarov I
Virology Unit, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.
Ann N Y Acad Sci. 1990;590:157-67. doi: 10.1111/j.1749-6632.1990.tb42218.x.
Infection of macrophages by intracellular parasites might modulate production of tumor necrosis factor (TNF) and prostaglandin E2 (PGE2), which, in turn, might have a profound effect on the outcome of the infection in vivo. In this study we examined in an in vitro system, the rickettsial yield in human monocyte-derived macrophages (MdM) and the PGE2 and TNF production by MdM infected with Rickettsia conorii RC, Casablanca strain) or Israeli spotted fever (ISF, G-212 strain). TNF and PGE2 were determined in the media of MdM infected with RC or ISF. TNF reached maximum levels 24 h post-infection and then declined, while PGE2 levels increased continuously during the infection up to 96 h post-infection. Addition of dexamethasone inhibited both TNF and PGE2 production and enhanced rickettsial yield in MdM. Inhibition of PGE2 production by indomethacin resulted in increased production of TNF from rickettsial-infected MdM, while addition of PGE2 caused partial inhibition of TNF production from infected MdM.
细胞内寄生虫感染巨噬细胞可能会调节肿瘤坏死因子（TNF）和前列腺素E2（PGE2）的产生，而这反过来可能会对体内感染的结果产生深远影响。在本研究中，我们在体外系统中检测了人单核细胞衍生巨噬细胞（MdM）中的立克次氏体产量，以及感染康氏立克次氏体（RC，卡萨布兰卡菌株）或以色列斑点热（ISF，G - 212菌株）的MdM产生PGE2和TNF的情况。在感染RC或ISF的MdM培养基中测定TNF和PGE2。TNF在感染后24小时达到最高水平，然后下降，而PGE2水平在感染期间持续升高，直至感染后96小时。添加地塞米松可抑制TNF和PGE2的产生，并提高MdM中立克次氏体的产量。吲哚美辛抑制PGE2的产生会导致立克次氏体感染的MdM中TNF产生增加，而添加PGE2会部分抑制感染的MdM中TNF的产生。
