Kellermayer M, Jobst K
Histochemistry. 1975 Jul 30;44(2):193-5. doi: 10.1007/BF00494081.
Monolayer HeLa culture nuclei isolated in situ with nonionic detergents remained attached to the glass as a "nuclear monolayer". Searching for nuclear fixing structures a fine continuous protein network was revealed around the isolated nuclei with fluorescence microscope after acridine orange staining.
用非离子去污剂原位分离的单层海拉细胞培养细胞核作为“核单层”仍附着在玻璃上。在用吖啶橙染色后,通过荧光显微镜在分离的细胞核周围发现了一个精细的连续蛋白质网络,以此来寻找核固定结构。