新形成的核糖体蛋白在HeLa细胞组分中的分布。
Distribution of newly formed ribosomal proteins in HeLa cell fractions.
作者信息
Warner J R
出版信息
J Cell Biol. 1979 Mar;80(3):767-72. doi: 10.1083/jcb.80.3.767.
Abstract
The distribution of newly formed ribosomal proteins between cytoplasmic, nucleoplasmic, and nucleolar fractions of HeLa cells was determined. All but a few of the newly formed ribosomal proteins were concentrated 10- to 50-fold in the nucleolus and two- to fivefold in the nucleoplasm. Nevertheless, substantial amounts were found in the cytoplasm. Pretreatment of cells with actinomycin D to deplete the nucleolar pool of ribosomal precursor RNA had no effect on the concentration of newly formed ribosomal proteins in the nucleus, but did lead to an increased amount in the nucleoplasm at the expense of the nucleolus.
摘要
测定了HeLa细胞胞质、核质和核仁组分中新合成核糖体蛋白的分布情况。除少数新合成的核糖体蛋白外，其余大部分在核仁中富集了10至50倍，在核质中富集了2至5倍。然而，在细胞质中也发现了大量的核糖体蛋白。用放线菌素D预处理细胞以耗尽核糖体前体RNA的核仁池，对细胞核中新合成核糖体蛋白的浓度没有影响，但确实导致核质中核糖体蛋白数量增加，而核仁中的数量相应减少。
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