Wexner Medical Center, The Arthur G. James Comprehensive Cancer Center and Richard J. Solove Research Institute, Department of Radiation Oncology, The Ohio State University , Columbus, Ohio , USA.
Int J Radiat Biol. 2013 Dec;89(12):1094-9. doi: 10.3109/09553002.2013.817703. Epub 2013 Jul 29.
To use NanoDot dosimeters to study the RS 2000 X-ray Biological Irradiator dosimetry characteristics and perform in vivo dosimetry for cell or small animal experiments.
We first calibrated the Landauer NanoDot(™) Reader by irradiating some NanoDot dosimeters with a set of known doses at specific positions defined by the irradiator. A group of five NanoDot dosimeters were placed at five specific positions where the dose rates were known and provided by the irradiator. Each group was irradiated for a set of times respectively. By correlating the readings of dosimeters with the given irradiated doses, we established the dose-reading relationship for the irradiator under the specific running condition. The established calibration curve was validated by exposing arbitrary known doses to a set of dosimeters, using the Landauer NanoDot(™) Reader to measure the doses, and then making the comparison between the two doses. To study the dose gradient of the X-ray inside the irradiated target (dose variation/cm), we placed dosimeters under different thicknesses of water-equivalent bolus and irradiated them, then measured the doses to determine the dose gradient.
Using the method described above, we were able to calibrate the Landauer InLight NanoDot(™) Reader and use NanoDot dosimeters to measure the actual doses delivered to the targets for the cell/small animal experiments that use the RS 2000 X-ray Biological Irradiator.
NanoDots are ideal dosimeters to use for in vivo dosimetry for cell/small animal irradiation experiments. The dose decrease inside the animal tissue is about 20% per cm.
使用 NanoDot 剂量计研究 RS 2000 X 射线生物辐照器的剂量学特性，并进行细胞或小动物实验的体内剂量测量。
我们首先使用一组已知剂量在辐照器特定位置（由辐照器定义）照射一组 NanoDot 剂量计，对 Landauer NanoDot（™）阅读器进行校准。将五组 NanoDot 剂量计放置在已知剂量率且由辐照器提供的五个特定位置。每组分别进行一组设定时间的照射。通过将剂量计的读数与给定的照射剂量相关联，我们在特定运行条件下建立了辐照器的剂量读数关系。通过将任意已知剂量暴露于一组剂量计，使用 Landauer NanoDot（™）阅读器测量剂量，然后对两个剂量进行比较，验证了所建立的校准曲线。为了研究辐照目标内的 X 射线剂量梯度（剂量变化/cm），我们在不同厚度的水等效块下放置剂量计并对其进行照射，然后测量剂量以确定剂量梯度。
使用上述方法，我们能够校准 Landauer InLight NanoDot（™）阅读器，并使用 NanoDot 剂量计测量使用 RS 2000 X 射线生物辐照器进行细胞/小动物实验时实际输送到靶标的剂量。
NanoDots 是用于细胞/小动物辐照实验体内剂量测量的理想剂量计。动物组织内的剂量下降约为每厘米 20%。
