Nöll Junior Research Group for Nanotechnology, Siegen University, Faculty IV, Department of Chemistry-Biology, Organic Chemistry, Adolf-Reichwein-Str. 2, 57068 Siegen, Germany.
Adv Healthc Mater. 2014 Jan;3(1):42-6. doi: 10.1002/adhm.201300056. Epub 2013 Jun 21.
The development of a reusable molecular beacon (MB)-based sensor for the label-free detection of specific oligonucleotides using surface plasmon fluorescence spectroscopy (SPFS) as the readout method is described. The MBs are chemisorbed at planar gold surfaces serving as fluorescence quenching units. Target oligonucleotides of 24 bases can be detected within a few minutes at high single-mismatch discrimination rates.
本文描述了一种可重复使用的分子信标（MB）传感器的开发，该传感器使用表面等离子体荧光光谱（SPFS）作为读出方法，用于对特定寡核苷酸进行无标记检测。MB 被化学吸附在平面金表面上，作为荧光猝灭单元。在几分钟内，可以以高单错配率检测到 24 个碱基的目标寡核苷酸。
