Laboratory of Photochemistry, Department of Drug Sciences, University of Catania, Viale Andrea Doria 6, I-95125 Catania (Italy).
Chem Asian J. 2013 Nov;8(11):2634-41. doi: 10.1002/asia.201300463. Epub 2013 Jun 20.
We report the design, preparation, and properties of a nanoaggregate formed in phosphate buffer solution by a porphyrin-β-cyclodextrin (β-CD) conjugate and a nitric oxide photodonor tailored to fit the β-CD cavity. The small nanoassembly with a diameter of about 13 nm exhibits the typical red fluorescence of the porphyrin chromophore. The empty cavity of the β-CD unit in the nanoaggregate is able to accommodate the NO photodonor, thereby forming a supramolecular bichromophoric aggregate with diameter of about 16 nm. This nanoconstruct preserves the fluorescence of the porphyrin core and is able to generate nitric oxide and singlet oxygen under illumination with visible light. The nanoassembly internalizes in melanoma cells, can be mapped therein by fluorescence microscopy, and induces a significant level of cell mortality, probably due to the combined action of reactive nitrogen oxide species (RNOS) and reactive oxygen species (ROS).
我们报告了一种纳米聚集体的设计、制备和性质，该聚集体由卟啉-β-环糊精（β-CD）缀合物和一氧化氮光供体在磷酸盐缓冲溶液中形成，该光供体经过精心设计以适应β-CD 空腔。这种直径约为 13nm 的小纳米组装体表现出卟啉发色团的典型红色荧光。β-CD 单元的空腔能够容纳 NO 光供体，从而形成直径约为 16nm 的超分子双生色聚集体。这种纳米结构保留了卟啉核心的荧光，并能够在可见光照射下生成一氧化氮和单线态氧。该纳米组装体在黑色素瘤细胞内化，并可通过荧光显微镜在其中进行定位，并诱导显著水平的细胞死亡，这可能是由于活性氮氧化物（RNOS）和活性氧（ROS）的共同作用。
