CycloLab Ltd., H-1097 Budapest, Illatos út 7, Hungary.
Biomater Sci. 2019 May 28;7(6):2272-2276. doi: 10.1039/c9bm00395a.
We report herein on a novel water-soluble β-cyclodextrin-branched polymer covalently integrating a fluorescein moiety and a nitric oxide (NO) photodonor within its macromolecular skeleton. Photoexcitation with visible light induces the parallel activation of the two chromophores, which results in the green fluorescence emission suitable for imaging accompanied by NO release for therapy. In fact, this polymer internalizes in squamous carcinoma cancer cells in vitro, visualized by fluorescence microscopy, and induces cell mortality as result of the NO photo-decaging. The non-covalent drug delivery capability of this new material is also demonstrated using a hydrophobic photosensitizer for photodynamic therapy as a probe.
我们在此报告了一种新型的水溶性β-环糊精支化聚合物，它将荧光素部分和一氧化氮（NO）光供体共价结合在其高分子骨架内。可见光激发可同时激活两个生色团，导致适合成像的绿色荧光发射，同时释放 NO 进行治疗。事实上，这种聚合物在体外可进入鳞状癌细胞并通过荧光显微镜进行可视化，NO 的光解笼导致细胞死亡。还使用光动力治疗的疏水性光敏剂作为探针证明了这种新材料的非共价药物递送能力。
