Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School and the Wyss Institute of Biologically Inspired, Engineering of Harvard University, Boston, MA 02115, USA; Coulter Department of Biomedical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Adv Healthc Mater. 2014 Jan;3(1):30-5. doi: 10.1002/adhm.201300110. Epub 2013 Jun 21.
Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.
使用经过改造的 sortase（eSrtA）突变体，可以快速一步将血栓调节蛋白与烷基胺衍生物（如叠氮化物、生物素和 PEG）进行修饰。本文证明了一种即时护理方案的可行性，即通过定点将叠氮化物-血栓调节蛋白固定在经过消毒的商用 ePTFE 血管移植物上，与商用肝素涂覆的移植物相比，在灵长类动物急性移植物血栓形成模型中，该移植物具有更好的抗血栓性。
