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酶促生物正交化学用于重组血栓调节蛋白的定点双修饰。

Chemoenzymatic bio-orthogonal chemistry for site-specific double modification of recombinant thrombomodulin.

机构信息

Department of Chemistry, Chemical and Biomedical Engineering, Cleveland State University, 2121 Euclid Ave, Cleveland, OH 44115 (USA).

出版信息

Chembiochem. 2014 Jan 3;15(1):42-6. doi: 10.1002/cbic.201300641. Epub 2013 Nov 7.

DOI:10.1002/cbic.201300641
PMID:24357004
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3926800/
Abstract

Best of both worlds: A one-pot strategy for site-specific PEGylation through strain-promoted alkyne-azide cycloaddition (SPAAC) and fluorescent labeling through sortase A-mediated ligation (SML) of recombinant thrombomodulin without prior chemical modification and without diminishing the protein activity has been developed.

摘要

两全其美

通过应变促进的叠氮化物-炔烃环加成（SPAAC）进行位点特异性聚乙二醇化和通过连接酶 A 介导的连接（SML）进行荧光标记的一锅法策略，无需预先进行化学修饰且不降低蛋白质活性，已被开发用于重组血栓调节蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/0eaebb757bc4/nihms553942f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/128f631b6edc/nihms553942f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/0afbf918f493/nihms553942f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/e2e35507573b/nihms553942f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/9992207edbfc/nihms553942f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/17a54bbacc03/nihms553942f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/0eaebb757bc4/nihms553942f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/128f631b6edc/nihms553942f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/0afbf918f493/nihms553942f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/e2e35507573b/nihms553942f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/9992207edbfc/nihms553942f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/17a54bbacc03/nihms553942f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e230/3926800/0eaebb757bc4/nihms553942f6.jpg

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Bioconjug Chem. 2012 Nov 21;23(11):2256-61. doi: 10.1021/bc300364z. Epub 2012 Oct 17.
2
Sortase-Mediated Transpeptidation for Site-Specific Modification of Peptides, Glycopeptides, and Proteins.用于肽、糖肽和蛋白质位点特异性修饰的分选酶介导的转肽作用。
J Carbohydr Chem. 2012;31(1):48-66. doi: 10.1080/07328303.2011.635251. Epub 2012 Jan 19.
3
End-point immobilization of recombinant thrombomodulin via sortase-mediated ligation.
Polymers (Basel). 2018 Feb 6;10(2):151. doi: 10.3390/polym10020151.
4
Layer-by-Layer Heparinization of the Cell Surface by Using Heparin-Binding Peptide Functionalized Human Serum Albumin.利用肝素结合肽功能化人血清白蛋白对细胞表面进行逐层肝素化。
Materials (Basel). 2018 May 20;11(5):849. doi: 10.3390/ma11050849.
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A versatile papaya mosaic virus (PapMV) vaccine platform based on sortase-mediated antigen coupling.一种基于分选酶介导的抗原偶联的通用木瓜花叶病毒（PapMV）疫苗平台。
J Nanobiotechnology. 2017 Jul 18;15(1):54. doi: 10.1186/s12951-017-0289-y.
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