Akdogan Elif, Cengiz Sevil, Yılmaz Mustafa, Sönmez Mehmet, Durmus Ahmet, Oval Ercüment, Omay Serdar Bedii
Department of Hematology, Recep Tayyip Erdogan University, Rize, Turkey.
Contemp Oncol (Pozn). 2013;17(1):83-7. doi: 10.5114/wo.2013.33780. Epub 2013 Mar 15.
Protein tyrosine phosphatases regulate physiological processes including growth, differentiation, metabolism and the cell cycle. Together with tyrosine kinases, they control the phosphorylation state of tyrosine residues of signaling proteins. An increased level of protein phosphorylation results in abnormal proliferation and many cancer types show a mutation or deletion of a protein tyrosine phosphatase gene. In this study we evaluated the protein tyrosine phosphatase activity in acute leukemia patients. Tyrosine phosphatase activity in bone marrow mononuclear cells of acute leukemia patients was measured using a tyrosine phosphatase assay system kit and compared with a control group. We found that tyrosine phosphatase activity in acute leukemia patients was high compared to the controls. According to subgroups of acute leukemia, tyrosine phosphatase activity in the AML-M2 subgroup was high compared to the controls. The effect of increased level of protein tyrosine phosphatase activity on leukemogenesis needs further evaluation. Studies in a large group of patients are needed to emphasize the importance of tyrosine phosphatase activity in acute leukemia patients.
蛋白质酪氨酸磷酸酶调节包括生长、分化、代谢和细胞周期在内的生理过程。它们与酪氨酸激酶一起，控制信号蛋白酪氨酸残基的磷酸化状态。蛋白质磷酸化水平升高会导致异常增殖，许多癌症类型都显示出蛋白质酪氨酸磷酸酶基因的突变或缺失。在本研究中，我们评估了急性白血病患者的蛋白质酪氨酸磷酸酶活性。使用酪氨酸磷酸酶检测系统试剂盒测量急性白血病患者骨髓单个核细胞中的酪氨酸磷酸酶活性，并与对照组进行比较。我们发现，与对照组相比，急性白血病患者的酪氨酸磷酸酶活性较高。根据急性白血病的亚组分类，AML-M2亚组中的酪氨酸磷酸酶活性高于对照组。蛋白质酪氨酸磷酸酶活性水平升高对白血病发生的影响需要进一步评估。需要对大量患者进行研究，以强调酪氨酸磷酸酶活性在急性白血病患者中的重要性。
