Karlsruhe Institute of Technology, 76021, Karlsruhe, Germany.
Langmuir. 2013 Jul 2;29(26):8355-62. doi: 10.1021/la400721c. Epub 2013 Jun 21.
Poly(ethylene glycol) (PEG) is one of the most extensively studied antifouling coatings due to its ability to reduce protein adsorption and improve biocompatibility. Although the use of PEG for antifouling coatings is well established, the stability and density of PEG layers are often inadequate to provide optimum antifouling properties. To improve on these shortcomings, we employed the stepwise construction of PEG layers onto a silicon surface. Acetylene-terminated alkyl monolayers were attached to nonoxidized crystalline silicon surfaces via a one-step hydrosilylation procedure with 1,8-nonadiyne. The acetylene-terminated surfaces were functionalized via a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction of the surface-bound alkynes with an azide to produce an amine terminated layer. The amine terminated layer was then further conjugated with PEG to produce an antifouling surface. The antifouling surface properties were investigated by testing adsorption of human serum albumin (HSA) and lysozyme (Lys) onto PEG layers from phosphate buffer solutions. Detailed characterization of protein fouling was carried out with X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) combined with principal component analysis (PCA). The results revealed no fouling of albumin onto PEG coatings whereas the smaller protein lysozyme adsorbed to a very low extent.
聚乙二醇（PEG）是研究最多的一种抗污涂层，因为它可以减少蛋白质吸附并提高生物相容性。尽管 PEG 已被广泛用于抗污涂层，但 PEG 层的稳定性和密度通常不足以提供最佳的抗污性能。为了克服这些缺点，我们采用逐步构建 PEG 层的方法在硅表面上进行。乙炔末端烷基单层通过一步硅氢化反应与 1,8-辛二炔连接到非氧化晶态硅表面。乙炔末端表面通过铜催化的叠氮-炔环加成（CuAAC）反应进行官能化，即将表面结合的炔烃与叠氮化物反应生成胺封端层。然后，胺封端层与 PEG 进一步共轭，生成抗污表面。通过从磷酸盐缓冲溶液中测试人血清白蛋白（HSA）和溶菌酶（Lys）在 PEG 层上的吸附，研究了抗污表面的性质。利用 X 射线光电子能谱（XPS）和飞行时间二次离子质谱（ToF-SIMS）结合主成分分析（PCA）对蛋白质污染进行了详细表征。结果表明，白蛋白不会在 PEG 涂层上发生污染，而较小的蛋白质溶菌酶吸附程度非常低。
