Department of Pulmonary Medicine, Aarhus University Hospital, Aarhus, Denmark.
Scand J Immunol. 2013 Oct;78(4):352-6. doi: 10.1111/sji.12085.
The function of cultured mast cells may depend on genetic or environmental influence on the stem cell donor. This study investigates whether asthma or atopy in the donor influenced the growth and sensitivity of mast cells cultured from patients with asthma and healthy controls under identical conditions. Mast cells were cultured from peripheral blood from twelve patients with an objectively confirmed asthma diagnosis and eight healthy subjects. During the last 2 weeks of culture, mast cells were incubated with IL-4 and 80 kU/l recombinant human IgE containing two clones (7% + 7%) specific for mite allergen Der p2. The sensitivity of IgE-mediated activation of mast cells was investigated as FcεRI-mediated upregulation of CD63. Ten subjects were atopic, defined as a positive skin prick test (>3 mm) to at least one of ten common allergens. After activation with recombinant Der p2, the maximum CD63 median fluorescence intensity was 20 456 ± 1640 (SE) for patients with asthma and 22,275 ± 1971 (SE) for controls (ns). The fraction of CD63 positive cells was 54.4% in patients with asthma and 48.4% in controls (ns). The allergen concentration inducing 50% of the maximal CD63 response was similar in patients with asthma [-0.4795 log ng/ml ± 0.092 (SE)] and controls (-0.6351 log ng/ml ± 0.083, ns) and in atopic and non-atopic subjects. When cultured, sensitized and activated under identical conditions, mast cells from allergic asthmatics and healthy controls respond similar. Activation of cultured mast cells appears to depend on culture conditions (IL-4, IgE) rather than on donor status as atopy and asthma.
培养的肥大细胞的功能可能取决于供体的遗传或环境影响。本研究调查供体中的哮喘或过敏是否影响在相同条件下培养的来自哮喘患者和健康对照者的肥大细胞的生长和敏感性。从 12 名经客观确认的哮喘诊断患者和 8 名健康受试者的外周血中培养肥大细胞。在培养的最后 2 周期间，将肥大细胞与 IL-4 和 80 kU/l 含有针对螨变应原 Der p2 的两个克隆（7%+7%）的重组人 IgE 一起孵育。通过 FcεRI 介导的 CD63 上调来研究 IgE 介导的肥大细胞激活的敏感性。10 名受试者为过敏体质，定义为对至少十种常见过敏原之一的皮肤点刺试验（>3 mm）呈阳性。用重组 Der p2 激活后，哮喘患者的最大 CD63 中荧光强度中位数为 20 456±1640（SE），而对照组为 22,275±1971（SE）（无统计学差异）。哮喘患者中 CD63 阳性细胞的分数为 54.4%，对照组为 48.4%（无统计学差异）。诱导最大 CD63 反应 50%的变应原浓度在哮喘患者[-0.4795 log ng/ml±0.092（SE）]和对照组[-0.6351 log ng/ml±0.083，无统计学差异]以及过敏和非过敏受试者中相似。在相同条件下培养、致敏和激活时，来自过敏性哮喘患者和健康对照者的肥大细胞的反应相似。培养的肥大细胞的激活似乎取决于培养条件（IL-4、IgE），而不是供体状态，如过敏和哮喘。
