Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, Sagamihara, Japan; Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
Biophys Chem. 2013 Dec 15;183:3-8. doi: 10.1016/j.bpc.2013.05.005. Epub 2013 May 29.
High hydrostatic pressure has a profound physiological impact on lipid membranes, primarily resulting in tighter packing and restriction of acyl-chain motion. To fulfill membrane protein functions in high-pressure environments, deep-sea organisms possess specialized cell membranes. Although the effects of high-pressure on model membranes have been investigated in great detail, high-pressure-induced structural changes in living cell membranes remain to be elucidated. Of the spectroscopic techniques available to date, fluorescence anisotropy measurement is a common useful method that provides information on dynamic membrane properties. This mini-review focuses on pressure-induced changes in natural cell membranes, analyzed by means of high-pressure time-resolved fluorescence anisotropy measurement (HP-TRFAM). Specifically, the role of eicosapentaenoic acid in deep-sea piezophiles is described in terms of the structural integrity of the membrane under high pressure.
高静水压力对脂膜有深远的生理影响，主要表现为酰链运动的紧密堆积和受限。为了在高压环境中实现膜蛋白的功能，深海生物拥有特殊的细胞膜。尽管已经详细研究了高压对模型膜的影响，但活细胞膜在高压下的结构变化仍有待阐明。在现有的光谱技术中，荧光各向异性测量是一种常见的有用方法，可以提供有关动态膜性质的信息。本篇综述聚焦于通过高压时间分辨荧光各向异性测量（HP-TRFAM）分析的天然细胞膜在高压下的变化。具体来说，文中描述了深海嗜压生物中二十碳五烯酸（EPA）在维持膜结构完整性方面的作用。
