CIIMAR - Centre for Marine Environmental Research, Rua dos Bragas, 289, 4050-123 Porto, Portugal.
Mar Pollut Bull. 2013 Aug 15;73(1):70-7. doi: 10.1016/j.marpolbul.2013.05.039. Epub 2013 Jun 19.
Sixty female flounder (Platichthys flesus) were collected in Autumn 2011, 15 from each of the following sampling sites: at the mouths of the Douro and Vistula Rivers, and at nearby open sea locations. The aim of the study was to assess several biomarkers in the two geographically distant regions. Hepatic EROD, GST, SOD, GPx, POx, LP; muscular AChE, BChE, LP; and branchial Na(+)/K(+)-ATPase were analysed. Moreover, BTI, PY, and three gross morphometric indices were calculated. The results were analysed with t-test, ANOVA, and PCA. Many differences were found between the open sea sites and the river mouths, mainly in Portugal, and between the two rivers. Salinity and pollution seem to be the main factors that affected the biomarkers. Effects of chronic pollution were observed at the river mouths, and an indication of a possible temporary exposure to pollutants was found at the open ocean site in Portugal.
2011 年秋季采集了 60 条雌性欧鲽（Platichthys flesus），每个采样点采集 15 条，分别位于杜罗河和维斯杜拉河口及附近的公海。本研究的目的是评估两个地理位置相距较远的地区的几种生物标志物。分析了肝 EROD、GST、SOD、GPx、POx、LP；肌肉 AChE、BChE、LP；以及鳃 Na(+)/K(+)-ATP 酶。此外，还计算了 BTI、PY 和三个大体形态指数。用 t 检验、方差分析和主成分分析对结果进行了分析。公海地点和河口之间，主要是在葡萄牙，以及两条河流之间，发现了许多差异。盐度和污染似乎是影响生物标志物的主要因素。在河口处观察到了慢性污染的影响，并且在葡萄牙的公海地点发现了可能暂时接触污染物的迹象。
