Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, Via Ponte don Melillo, 84084 Fisciano (SA), Italy.
Food Chem. 2013 Nov 15;141(2):745-53. doi: 10.1016/j.foodchem.2013.03.041. Epub 2013 Mar 21.
Liquid chromatography electro-spray tandem mass spectrometry (LC-ESI/MS/MS) was applied to the determination of sweet glycosides (steviol glycosides), and toxic aglycon steviol in 24 samples of Stevia rebaudiana (Bertoni) aerial parts, which had been experimentally cultivated in Italy, although derived from seeds of different geographical origin. On the basis of the specific fragmentation of these compounds, an LC-MS/MS method was developed with the aim of quantifying analytes in plant material. Although toxic steviol was not detectable in all the samples, the samples with the highest levels of steviol glycosides were identified. Analysis of the different samples revealed that they were good quality samples, quality being directly linked to the presence of sweet glycosides in the plants cultivated in Italy, although there were differences in the content of these compounds according to the origin of the seeds, and in particular, a major concentration of compounds with major sweetness activity and minor toxicity was found in the population coming from Brazil (for example: sample 10, stevioside content 15.74±2.0% p/p and rebaudioside A content 3.09±0.39% p/p of dried plant). Finally, based on this metabolomic targeted approach, the results obtained for the samples were treated by Principal Component Analysis, identifying specific genotypic differences based on the geographic origin of the seeds.
液相色谱-电喷雾串联质谱法（LC-ESI/MS/MS）被应用于甜糖苷（甜菊糖苷）和有毒糖苷 Steviol 的测定，对在意大利实验种植的 24 份甜叶菊（Bertoni）地上部分样本进行了检测，这些样本来自不同地理起源的种子。基于这些化合物的特异性碎片，建立了一种 LC-MS/MS 方法，旨在对植物材料中的分析物进行定量。虽然在所有样本中都检测不到有毒的 Steviol，但确定了甜菊糖苷含量最高的样本。对不同样本的分析表明，它们是优质的样本，质量与在意大利种植的甜菊糖苷的存在直接相关，尽管根据种子的来源，这些化合物的含量存在差异，特别是在来自巴西的种群中发现了具有主要甜度活性和较小毒性的化合物的主要浓度（例如：样本 10，甜菊糖苷含量 15.74±2.0% p/p，莱鲍迪苷 A 含量 3.09±0.39% p/p 的干燥植物）。最后，基于这种代谢组学靶向方法，对样本的结果进行主成分分析处理，根据种子的地理起源识别出特定的基因型差异。
