KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Food Chem. 2013 Nov 15;141(2):1369-77. doi: 10.1016/j.foodchem.2013.04.012. Epub 2013 Apr 18.
The ginsenoside Rg3(S), which is one of the exceptional components of Korean red ginseng extract, has been known to have anti-cancer, anti-metastatic, and anti-obesity effects. An enzymatic bioconversion method was developed to obtain the ginsenoside Rg3(S) with a high specificity, yield, and purity. Two glycoside hydrolases (BglBX10 and Abf22-3) were employed to produce Rg3(S) as a 100g unit. The conversion reaction transformed ginsenoside Rc to Rd using Abf22-3, followed by Rb1 and Rd to Rg3(S), using BglBX10. It was performed in a 10L jar fermenter at pH 6.0 and 37°C for 24h, with a high concentration of 50mg/ml of purified ginsenoside mixture obtained from ginseng roots. Finally, 144g of Rg3(S) was produced from 250g of root extract with 78±1.2% chromatographic purity. These results suggest that this enzymatic method would be useful in the preparation of ginsenoside Rg3(S) for the functional food and pharmaceutical industries.
稀有人参皂苷 Rg3(S)是红参提取物的一种特殊成分，具有抗癌、抗转移和抗肥胖作用。本研究开发了一种酶法生物转化方法，以获得高特异性、高收率和高纯度的稀有人参皂苷 Rg3(S)。采用两种糖苷水解酶（BglBX10 和 Abf22-3）以 100g 单位生产 Rg3(S)。转化反应首先使用 Abf22-3 将人参皂苷 Rc 转化为 Rd，然后使用 BglBX10 将 Rb1 和 Rd 转化为 Rg3(S)。在 pH 6.0 和 37°C 的 10L 搅拌式发酵罐中进行 24 小时反应，使用浓度为 50mg/ml 的人参根中提取的纯化人参皂苷混合物。最终，从 250g 根提取物中制备得到了 144g 稀有人参皂苷 Rg3(S)，其色谱纯度为 78±1.2%。这些结果表明，该酶法适合用于功能性食品和制药行业中稀有人参皂苷 Rg3(S)的制备。
