Chen Linhao, Pang Yaru, Luo Yan, Cheng Xu, Lv Bo, Li Chun
Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Ministry of Industry and Information Technology Institute of Biochemical Engineering School of Chemistry and Chemical Engineering Beijing Institute of Technology Beijing P. R. China.
Key Lab for Industrial Biocatalysis Ministry of Education Department of Chemical Engineering Tsinghua University Beijing P. R. China.
Eng Life Sci. 2021 Jun 10;21(11):724-738. doi: 10.1002/elsc.202100014. eCollection 2021 Nov.
The production of plant terpenoids through biotransformation has undoubtedly become one of the research hotspots, and the continuous upgrading of the corresponding downstream technology is also particularly important. Downstream technology is the indispensable technical channel for the industrialization of plant terpenoids. How to efficiently separate high-purity products from complex microbial fermentation broths or enzyme-catalyzed reactions to achieve high separation rates, high returns and environmental friendliness has become the focus of research in recent years. This review mainly introduces the common separation methods of plant terpenoids based on biotransformation from the perspectives of engineering strain construction, unit separation technology, product properties and added value. Then, further attention was paid to the application prospects of intelligent cell factories and control in the separation of plant terpenoids. Finally, some current challenges and prospects are proposed, which provide possible directions and guidance for the separation and purification of terpenoids and even industrialization.
通过生物转化生产植物萜类化合物无疑已成为研究热点之一，相应下游技术的不断升级也尤为重要。下游技术是植物萜类化合物产业化不可或缺的技术渠道。如何从复杂的微生物发酵液或酶催化反应中高效分离出高纯度产品，以实现高分离率、高回报和环境友好，已成为近年来的研究重点。本文综述主要从工程菌株构建、单元分离技术、产品性质和附加值等角度介绍基于生物转化的植物萜类化合物常见分离方法。进而关注了智能细胞工厂及控制在植物萜类化合物分离中的应用前景。最后提出了当前的一些挑战与展望，为萜类化合物的分离纯化乃至产业化提供了可能的方向和指导。
