Department of Pathology and Institute of Oncology, Fujian Medical University, Fuzhou 350004, China.
Hum Pathol. 2013 Aug;44(8):1681-7. doi: 10.1016/j.humpath.2013.04.007. Epub 2013 Jun 20.
Microsomal prostaglandin E2 synthase-1 (mPGES1), an inducible enzyme similar to cyclooxygenase-2, functions downstream of cyclooxygenase-2 in the synthesis of prostaglandin E2. It contributes to carcinogenesis in a variety of tumors. Here, mPGES1 expression was assessed using immunohistochemistry of tissue microarrays containing a total of 100 hepatocellular carcinoma (HCC) tissue samples, 100 peritumoral liver tissue samples, and 13 normal liver tissue samples. The expression of mPGES1 was significantly increased in the HCC tissue samples (P < .001), relative to normal liver tissue. Second, there was a significant positive correlation between mPGES1 expression and the Barcelona Clinic Liver Cancer stage (P < .001) in HCC tissue samples. This correlation was also observed with encapsulation (P = .004) and portal vein thrombosis (P < .001). In addition, the lentiviral vector (Lv-mPGES1-shRNA), which down-regulates mPGES1, inhibited tumor growth in an HCC animal model. Taken together, mPGES1 expression was associated with multiple malignant characteristics and enhanced tumorigenesis in HCC and may serve as an important clinical and pharmacologic biomarker.
微粒体前列腺素 E2 合酶-1（mPGES1）是一种与环氧化酶-2 相似的诱导酶，在前列腺素 E2 的合成中位于环氧化酶-2 的下游。它在多种肿瘤的癌变中起作用。在这里，使用包含总共 100 个肝细胞癌（HCC）组织样本、100 个肿瘤周围肝组织样本和 13 个正常肝组织样本的组织微阵列的免疫组织化学评估了 mPGES1 的表达。与正常肝组织相比，mPGES1 在 HCC 组织样本中的表达显著增加（P<0.001）。其次，在 HCC 组织样本中，mPGES1 的表达与巴塞罗那临床肝癌分期（P<0.001）之间存在显著正相关。在 HCC 组织样本中也观察到与包膜（P=0.004）和门静脉血栓形成（P<0.001）的相关性。此外，下调 mPGES1 的慢病毒载体（Lv-mPGES1-shRNA）抑制了 HCC 动物模型中的肿瘤生长。综上所述，mPGES1 的表达与多种恶性特征相关，并增强了 HCC 的肿瘤发生，可能成为重要的临床和药理生物标志物。
