Weyand E H, Patel S, LaVoie E J, Cho B, Harvey R G
Rutgers University, College of Pharmacy, Piscataway, NJ 08855-0789.
Cancer Lett. 1990 Jul 31;52(3):229-33. doi: 10.1016/0304-3835(90)90191-y.
The tumor-initiating activities of benzo[a]fluoranthene (BaF), benzo[b]fluoranthene (BbF), naphtho[1,2-b]fluoranthene (NbF) and naphtho[2,1-a]fluoranthene (NaF) were evaluated on the skin of female CD-1 mice. Each of these polycyclic aromatic hydrocarbons was assayed at total initiation doses of 1.0 and 4.0 mumol/mouse. These hydrocarbons were applied in 10 subdoses administered every other day. Promotion commenced 10 days after the last initiator dose and consisted of thrice weekly application of 2.5 micrograms of tetradecanoylphorbol acetate for 20 weeks. BbF was the most potent tumor initiator inducing a 100% incidence of tumor-bearing mice with an average of 8.5 tumors per mouse at a total initiator dose of 1.0 mumol. NaF was slightly more active as a tumor initiator than either NbF or BaF. NaF induced a 90% incidence of tumor-bearing mice with an average of 5.9 tumors per mouse at a total initiator dose of 1.0 mumol. BaF and NbF at a total initiator dose of 4.0 mumol exhibited similar tumor-initiating activity with both inducing a 90% incidence of tumor-bearing mice with an average of 4.3 and 6.6 tumors per mouse, respectively. However, at a total initiator dose of 1.0 mumol, BaF and NbF induced a 95% and 65% incidence of tumor-bearing mice with an average of 3.3 and 2.5 tumors per mouse, respectively.
在雌性CD-1小鼠的皮肤上评估了苯并[a]荧蒽（BaF）、苯并[b]荧蒽（BbF）、萘并[1,2-b]荧蒽（NbF）和萘并[2,1-a]荧蒽（NaF）的肿瘤起始活性。每种多环芳烃均以1.0和4.0 μmol/小鼠的总起始剂量进行测定。这些碳氢化合物以10个分剂量每隔一天给药一次。在最后一次起始剂给药10天后开始促进阶段，包括每周三次涂抹2.5微克十四酰佛波醇乙酸酯，持续20周。BbF是最有效的肿瘤起始剂，在总起始剂量为1.0 μmol时，诱导100%的荷瘤小鼠发生率，平均每只小鼠有8.5个肿瘤。NaF作为肿瘤起始剂的活性略高于NbF或BaF。在总起始剂量为1.0 μmol时，NaF诱导90%的荷瘤小鼠发生率，平均每只小鼠有5.9个肿瘤。总起始剂量为4.0 μmol时，BaF和NbF表现出相似的肿瘤起始活性，两者均诱导90%的荷瘤小鼠发生率，平均每只小鼠分别有4.3和6.6个肿瘤。然而，在总起始剂量为1.0 μmol时，BaF和NbF分别诱导95%和65%的荷瘤小鼠发生率，平均每只小鼠分别有3.3和2.5个肿瘤。
