Himeno T, Watanabe N, Yamauchi N, Maeda M, Tsuji Y, Okamoto T, Neda H, Niitsu Y
Department of Internal Medicine (Section 4), Sapporo Medical College, Japan.
Cancer Res. 1990 Aug 15;50(16):4941-5.
Based on the finding that expression of endogenous tumor necrosis factor (TNF) which is not detected in TNF-susceptible cells was observed in TNF-resistant cells, the assumption was made that endogenous TNF may be a protective protein against the cytotoxic activity of TNF. In order to confirm this possibility, we investigated the relationship between expression of endogenous TNF and TNF susceptibility by using the gene transfection method. When L-M, TNF-highly sensitive murine fibrosarcoma cells were transfected with a human TNF gene, the stable transfectants expressed endogenous TNF and acquired resistance to TNF. Conversely, when endogenous TNF synthesis was inhibited by introducing an antisense TNF gene into HeLa, TNF-less sensitive human cervical cancer cells, the sensitivity was enhanced. These findings indicate that endogenous TNF is one of the protective factors against the cytotoxic activity of TNF.
基于在对肿瘤坏死因子（TNF）敏感的细胞中未检测到内源性TNF表达，而在对TNF耐药的细胞中观察到该表达这一发现，人们推测内源性TNF可能是一种针对TNF细胞毒性活性的保护蛋白。为了证实这种可能性，我们通过基因转染方法研究了内源性TNF表达与TNF敏感性之间的关系。当用人类TNF基因转染对TNF高度敏感的小鼠纤维肉瘤细胞L-M时，稳定转染子表达内源性TNF并获得了对TNF的抗性。相反，当通过将反义TNF基因导入对TNF不太敏感的人宫颈癌细胞HeLa中来抑制内源性TNF合成时，其敏感性增强。这些发现表明，内源性TNF是针对TNF细胞毒性活性的保护因子之一。
