转染肿瘤坏死因子基因的人肿瘤浸润淋巴细胞可增强对自体肿瘤细胞的细胞毒性。
Human tumor-infiltrating lymphocytes transfected with tumor necrosis factor gene could augment cytotoxicity to autologous tumor cells.
作者信息
Itoh Y, Kohgo Y, Watanabe N, Kanisawa Y, Sakamaki S, Takahashi M, Hirayama Y, Ono H, Himeno T, Niitsu Y
机构信息
Department of Internal Medicine (Section 4), Sapporo Medical College.
出版信息
Jpn J Cancer Res. 1991 Nov;82(11):1203-6. doi: 10.1111/j.1349-7006.1991.tb01781.x.
Abstract
Human tumor-infiltrating lymphocytes (TILs) derived from pleural or ascitic fluid were incubated with recombinant interleukin 2 and transfected with human tumor necrosis factor (TNF) alpha gene by the lipofection procedure. The resulting TILs secreted significant amounts of TNF in the culture supernatant and exhibited cytotoxicity against established cell lines, such as K562 and Daudi, and autologous tumor cells. The TNF gene-transfected TILs exhibited an augmented killing of autologous tumor cells.
摘要
将源自胸水或腹水的人肿瘤浸润淋巴细胞（TILs）与重组白细胞介素2一起孵育，并通过脂质转染法用人类肿瘤坏死因子（TNF）α基因进行转染。所得的TILs在培养上清液中分泌大量TNF，并对诸如K562和Daudi等已建立的细胞系以及自体肿瘤细胞表现出细胞毒性。转染了TNF基因的TILs对自体肿瘤细胞的杀伤作用增强。
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Recombinant human TNF induces production of granulocyte-monocyte colony-stimulating factor.重组人肿瘤坏死因子可诱导粒细胞-单核细胞集落刺激因子的产生。
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Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1.肿瘤坏死因子（恶病质素）是一种内源性热原，可诱导白细胞介素1的产生。
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