Best A J, Das P K, Patel H R, Van Noorden C J
Laboratory of Cell Biology and Histology, University of Amsterdam, The Netherlands.
Cancer Res. 1990 Aug 15;50(16):5112-8.
It was found to be possible to distinguish malignant cells from normal cells by using an oxygen-sensitive tetrazolium salt (neotetrazolium) for the histochemical demonstration of glucose-6-phosphate dehydrogenase activity in cryostat sections of human colon. We have studied 12 cases of established adenocarcinoma of the colon in addition to 4 of ulcerative colitis and 4 of adenomatous polyposis (polyposis coli). In a nitrogen atmosphere the activities of malignant and normal cells were similar. However, after incubation in an atmosphere of pure oxygen, only malignant cells gave a positive reaction after 5 min. Three of the four cases of adenomatous polyposis gave a positive reaction for glucose-6-phosphate dehydrogenase activity in oxygen in a manner similar to that of specimens with severe dysplasia. In general, positive foci were histologically indistinguishable from the neighboring tubuli. However, foci of severely dysplastic epithelium usually showed a positive reaction. All three patients eventually developed clear-cut severe dysplasia. The other patient, who showed a negative reaction in oxygen, was diagnosed after 3 years as not suffering from dysplasia. All cases of ulcerative colitis gave a reaction in oxygen comparable with that of normal cells. Therefore, the areas with a positive reaction are considered to be either in the process of malignant transformation or malignant. An explanation for the oxygen insensitivity of cancer cells appeared to be a decrease in the activity of superoxide dismutase (EC 1.15.1.1), as addition of exogenous superoxide dismutase to malignant cells caused a normal reaction. We wish to suggest that this test in combination with the routine histology may be exploited for the diagnosis of polyps in premalignant conditions.
通过使用一种对氧敏感的四氮唑盐（新四氮唑）对人结肠冰冻切片中葡萄糖-6-磷酸脱氢酶活性进行组织化学显示，发现有可能区分恶性细胞和正常细胞。除了4例溃疡性结肠炎和4例腺瘤性息肉病（结肠息肉病）外，我们还研究了12例确诊的结肠腺癌病例。在氮气气氛中，恶性细胞和正常细胞的活性相似。然而，在纯氧气氛中孵育后，仅恶性细胞在5分钟后呈现阳性反应。4例腺瘤性息肉病病例中有3例在氧气中对葡萄糖-6-磷酸脱氢酶活性呈现阳性反应，其方式与重度发育异常标本相似。一般来说，阳性灶在组织学上与相邻小管无法区分。然而，重度发育异常上皮灶通常呈现阳性反应。所有这3例患者最终都发展为明确的重度发育异常。另1例患者在氧气中呈现阴性反应，3年后被诊断为无发育异常。所有溃疡性结肠炎病例在氧气中的反应与正常细胞相当。因此，呈现阳性反应的区域被认为要么处于恶性转化过程中，要么就是恶性的。癌细胞对氧不敏感的一个解释似乎是超氧化物歧化酶（EC 1.15.1.1）活性降低，因为向恶性细胞中添加外源性超氧化物歧化酶会导致正常反应。我们希望提出，结合常规组织学的这项检测可用于诊断癌前状态下的息肉。
