Van Driel B E, De Goeij A F, Song J Y, De Bruïne A P, Van Noorden C J
Academic Medical Centre, University of Amsterdam, Laboratory of Cell Biology and Histology, The Netherlands.
J Pathol. 1997 Aug;182(4):398-403. doi: 10.1002/(SICI)1096-9896(199708)182:4<398::AID-PATH869>3.0.CO;2-X.
The effects of oxygen on the quantitative histochemical assay to detect glucose-6-phosphate dehydrogenase (G6PDH) activity based on neotetrazolium reduction were studied in the different stages of carcinogenesis in the colon. Normal and hyperplastic epithelium, mucosae of patients with active Crohn's disease, and adenomas and adenocarcinomas of the colon were used. Epithelium of normal and inflamed mucosa, and hyperplastic epithelium, showed a residual G6PDH activity (RA) in oxygen that was always less than 20 per cent of the activity in the absence of oxygen. In adenomas and in dysplastic epithelia adjacent to carcinomas, the RA was significantly higher than that in normal epithelium, but significantly lower than that in adenocarcinomas. The RA of adenomas never exceeded 35 per cent. The RA of adenocarcinomas was on average 53 per cent and always higher than 20 per cent. When 35 per cent was used as a cut-off level, the sensitivity of RA to diagnose malignancy was 96.5 per cent. In a parallel study, a mouse model was used in which colon carcinomas and their precursors were induced chemically. Development of oxygen insensitivity during chemically induced carcinogenesis showed a pattern similar to that observed in the human. In conclusion, the test to determine RA is a useful tool for the detection of malignant mucosa in the colon. The test is particularly helpful in addition to histopathology for the detection of small lesions and the early stages of cancer.
在结肠癌变的不同阶段，研究了氧气对基于新四氮唑还原法检测葡萄糖 - 6 - 磷酸脱氢酶（G6PDH）活性的定量组织化学分析的影响。使用了正常和增生性上皮、活动性克罗恩病患者的黏膜以及结肠腺瘤和腺癌。正常和炎症黏膜的上皮以及增生性上皮在有氧条件下显示出残余G6PDH活性（RA），该活性始终低于无氧条件下活性的20%。在腺瘤以及癌旁发育异常的上皮中，RA显著高于正常上皮，但显著低于腺癌。腺瘤的RA从未超过35%。腺癌的RA平均为53%，且始终高于20%。以35%作为临界值时，RA诊断恶性肿瘤的敏感性为96.5%。在一项平行研究中，使用了化学诱导结肠癌及其前体的小鼠模型。化学诱导癌变过程中对氧气不敏感的发展呈现出与人类观察到的相似模式。总之，测定RA的试验是检测结肠恶性黏膜的有用工具。除组织病理学外，该试验对检测小病变和癌症早期特别有帮助。
