Department Pharmacology, School of Pharmacy, University of Seville, Spain.
J Ethnopharmacol. 2013 Aug 26;149(1):140-7. doi: 10.1016/j.jep.2013.06.013. Epub 2013 Jun 19.
Abarema cochliacarpos (Gomes) Barneby and Grimes (Fabaceae), known by the vulgar name of Babatenã, has been traditionally used in Northeast Brazil, as an anti-inflammatory remedy. Previous studies have demonstrated its anti-inflammatory and antiulcer effects in skin lesion, alcohol gastric ulcer and acute and chronic colitis.
The present study was designed to evaluate the antioxidant and anti-inflammatory effects of the butanolic fraction from A. cochliacarpos (BFAC) and its major flavonoid, (+)-catechin, in LPS-stimulated murine peritoneal macrophages. Moreover, we studied the role of mitogen-activated protein kinase (MAPK)s and NF-kB signaling pathways possibly involved in the beneficial effects.
The quantification of the extract was carried out by ultra-performance liquid chromatography analysis. Cell viability was determined using SRB assay. Nitric oxide (NO) production was analyzed by Griess method and intracellular reactive oxygen species (ROS) by fluorescence analysis. In addition, cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS) expression, MAPK activation and IkappaBalpha (IKBα) degradation, were determined by Western blot.
After BFAC characterization, (+)-catechin was revealed as its major constituent. Both BFAC and (+)-catechin, exerted significant anti-oxidant and anti-inflammatory effects inhibiting LPS-induced intracellular ROS and NO production in peritoneal macrophages. Additionally, the extract but also its major component reduced pro-inflammatory proteins expression probably through c-Jun N-terminal kinase and p38 MAPK signaling pathways.
These data suggest that the beneficial effects of BFAC might be mediated, at least in part, by the presence of (+)-catechin. Conclusively our findings confirm the potential of A. cochliacarpos as a new therapeutic strategy for the management of inflammatory and oxidative stress-related diseases.
Abarema cochliacarpos（Gomes）Barneby 和 Grimes（豆科），俗称 Babatenã，在巴西东北部被传统用于作为抗炎药物。先前的研究表明，它具有抗炎和抗溃疡作用，可用于皮肤损伤、酒精性胃溃疡和急性及慢性结肠炎。
本研究旨在评估 Abarema cochliacarpos 的正丁醇提取物（BFAC）及其主要类黄酮（+）-儿茶素对 LPS 刺激的小鼠腹腔巨噬细胞的抗氧化和抗炎作用。此外，我们研究了丝裂原激活蛋白激酶（MAPK）和 NF-kB 信号通路可能参与的有益作用。
通过超高效液相色谱分析对提取物进行定量。使用 SRB 测定法测定细胞活力。通过格里斯法分析一氧化氮（NO）的产生，通过荧光分析测定细胞内活性氧（ROS）。此外，通过 Western blot 测定环加氧酶（COX-2）和诱导型一氧化氮合酶（iNOS）的表达、MAPK 激活和 IkappaBalpha（IKBα）降解。
BFAC 表征后，发现（+）-儿茶素为其主要成分。BFAC 和（+）-儿茶素均能显著抑制 LPS 诱导的腹腔巨噬细胞内 ROS 和 NO 的产生，发挥抗氧化和抗炎作用。此外，该提取物及其主要成分还能降低促炎蛋白的表达，可能通过 c-Jun N-末端激酶和 p38 MAPK 信号通路。
这些数据表明，BFAC 的有益作用至少部分是由（+）-儿茶素介导的。综上所述，我们的研究结果证实了 Abarema cochliacarpos 作为治疗炎症和氧化应激相关疾病的新治疗策略的潜力。
