Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
FEBS Lett. 2013 Aug 2;587(15):2448-54. doi: 10.1016/j.febslet.2013.06.015. Epub 2013 Jun 19.
To identify potential biomarkers associated with Alzheimer's disease (AD)-like neuropathologies in the murine brain, we conducted proteomic analyses of neocortices from TgCRND8 mice. Here we found that phosphoprotein enriched in astrocytes 15 kDa (PEA-15) is expressed at higher levels in the neocortical proteomes from 6-month old TgCRND8 mice, as compared to non-transgenic mice. Immunostaining for PEA-15 revealed reactive astrocytes associated with the neocortical amyloid plaques in TgCRND8 mice and in post-mortem human AD brains. This is the first report of increased phosphoprotein enriched in astrocytes (PEA-15) expression in reactive astrocytes of an AD mouse model and human AD brains.
为了鉴定与阿尔茨海默病（AD）样神经病理学相关的潜在生物标志物，我们对 TgCRND8 小鼠的大脑新皮层进行了蛋白质组学分析。在这里，我们发现磷酸蛋白富含星形胶质细胞 15 kDa（PEA-15）在 6 个月大的 TgCRND8 小鼠的新皮层蛋白质组中表达水平更高，而非转基因小鼠。PEA-15 的免疫染色显示与 TgCRND8 小鼠和死后人类 AD 大脑中的新皮层淀粉样斑块相关的反应性星形胶质细胞。这是在 AD 小鼠模型和人类 AD 大脑的反应性星形胶质细胞中增加磷酸蛋白富含星形胶质细胞（PEA-15）表达的第一个报告。
