State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, China.
Insect Biochem Mol Biol. 2013 Sep;43(9):879-87. doi: 10.1016/j.ibmb.2013.06.004. Epub 2013 Jun 20.
Juvenile hormone (JH) is the primary hormone controlling vitellogenesis and oocyte maturation in the migratory locust Locusta migratoria, an evolutionarily primitive insect species with panoistic ovaries. However, molecular mechanisms of locust oogenesis remain unclear and the role of microRNA (miRNA) in JH mediated locust vitellogenesis and oocyte maturation has not been explored. Using miRNA sequencing and quantification with small RNA libraries derived from fat bodies of JH-deprived versus JH analog-exposed female adult locusts, we have identified 83 JH up-regulated and 60 JH down-regulated miRNAs. QRT-PCR validation has confirmed that transcription of selected miRNAs responded to JH administration and correlated with changes in endogenous hemolymph JH titers. Depletion of Argonaute 1 (Ago1), a key regulator of miRNA biogenesis and function by RNAi in female adult locusts dramatically decreased the expression of vitellogenin (Vg) and severely impaired follicular epithelium development, terminal oocyte maturation and ovarian growth. Our data indicate that Ago1 and Ago1-dependent miRNAs play a crucial role in locust vitellogenesis and egg production.
保幼激素（JH）是控制飞蝗（Locusta migratoria）卵黄发生和卵母细胞成熟的主要激素，飞蝗是一种具有多囊卵巢的进化原始昆虫物种。然而，蝗蝻卵发生的分子机制尚不清楚，并且 miRNA（miRNA）在 JH 介导的飞蝗卵黄发生和卵母细胞成熟中的作用尚未得到探索。使用 miRNA 测序和从小体组织衍生的小 RNA 文库进行定量，我们已经鉴定出 83 个 JH 上调和 60 个 JH 下调的 miRNA。qRT-PCR 验证已经证实，选定的 miRNA 的转录对 JH 给药有反应，并与内源性血淋巴 JH 滴度的变化相关。通过 RNAi 在雌性成蝗中耗尽 Argonaute 1（Ago1），这是 miRNA 生物发生和功能的关键调节剂，可显著降低卵黄蛋白原（Vg）的表达，并严重损害滤泡上皮发育、卵母细胞成熟和卵巢生长。我们的数据表明，Ago1 和 Ago1 依赖性 miRNA 在飞蝗卵黄发生和产卵中发挥着关键作用。
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