Miyake Y, Shigetoshi M, Kojima M
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Chem Pharm Bull (Tokyo). 1990 Apr;38(4):951-5. doi: 10.1248/cpb.38.951.
Each testosterone [125I]iodinated histamine derivative where [125I]iodinated histamines were linked to respective 15 alpha- and 15 beta-carboxymethyltestosterone (15 alpha- and 15 beta-CMT), testosterone-3-(O-carboxymethyl)oxime (T-3-CMO) and testosterone-17 beta-hemisuccinate (T-17-HS) were tested for their usefulness as radiotracers in testosterone immunoassay. In the use of anti-15 alpha- and 15 beta-CMT antisera produced in rabbits against 15 alpha- and 15 beta-CMT-bovine serum albumin (BSA) conjugates, the antisera with 15 alpha- and 15 beta-CMT-[125I]iodinated tracers showed low sensitivity and somewhat low specificity in comparison with those of the antisera with tritiated testosterone (T-3H). On the other hand, the antisera with T-3-CMO-[125I]iodinated tracer showed high sensitivity but low specificity for 5 alpha-dihydrotestosterone (5 alpha-DHT) in comparison with T-3H. The T-17-HS-[125I]iodinated tracer was not bound to the antisera. In the use of anti-15 alpha- and 15 beta-CMT antisera produced in rabbits by pretreatment with 15 alpha-carboxymethyl-5 alpha-DHT linked to a copolymer of D-glutamic acid and D-lysine followed by immunization with 15 alpha- and 15 beta-CMT-BSA, the antisera with homologous [125I]iodinated tracer showed high sensitivity and specificity.
对每种睾酮[¹²⁵I]碘化组胺衍生物进行了测试，这些衍生物中[¹²⁵I]碘化组胺分别与15α-和15β-羧甲基睾酮（15α-和15β-CMT）、睾酮-3-(O-羧甲基)肟（T-3-CMO）和睾酮-17β-半琥珀酸酯（T-17-HS）相连，以评估其作为睾酮免疫测定放射性示踪剂的实用性。在用兔制备的抗15α-和15β-CMT抗血清，针对15α-和15β-CMT-牛血清白蛋白（BSA）偶联物，与用氚化睾酮（T-3H）的抗血清相比，含15α-和15β-CMT-[¹²⁵I]碘化示踪剂的抗血清显示出低灵敏度和稍低的特异性。另一方面，含T-3-CMO-[¹²⁵I]碘化示踪剂的抗血清与T-3H相比，对5α-二氢睾酮（5α-DHT）显示出高灵敏度但低特异性。T-17-HS-[¹²⁵I]碘化示踪剂不与抗血清结合。在用与D-谷氨酸和D-赖氨酸共聚物相连的15α-羧甲基-5α-DHT预处理兔后，再用15α-和15β-CMT-BSA免疫制备的抗15α-和15β-CMT抗血清中，含同源[¹²⁵I]碘化示踪剂的抗血清显示出高灵敏度和特异性。
