来自各种生物来源的中性和磷脂甘油醚的色谱分析。

Chromatographic analysis of the neutral and phosphatide glyceryl ethers from various biological sources.

作者信息

Hack M H, Helmy F M

出版信息

J Chromatogr. 1975 Apr 9;107(1):155-66. doi: 10.1016/s0021-9673(00)82758-2.

Abstract

A method of analysis is described which permits the facile evaluation of the neutral glyceryl ether lipids (mild alkaline hydrolysis) and total glyceryl ether lipid (Vitride reduction) thereby allowing an assessment of the phosphatide contribution. The alkyl and alk-enyl glyceryl ethers are chromatographically resolvable on Gelman glass-fiber type SG and Whatman SG-81 and detected by the periodic acid-Schiff and plasmal reaction, respectively. The attending problems and interpretation are discussed with examples from a number of unicellular organisms and animal tissues.

摘要

本文描述了一种分析方法，该方法能够轻松评估中性甘油醚脂质（温和碱性水解）和总甘油醚脂质（红铝还原），从而评估磷脂的贡献。烷基和烯基甘油醚在Gelman玻璃纤维SG型和Whatman SG - 81上可通过色谱分离，分别用高碘酸 - 席夫反应和醛反应检测。文中结合许多单细胞生物和动物组织的实例讨论了相关问题及解释。