Pugh E L, Kates M, Hanahan D J
J Lipid Res. 1977 Nov;18(6):710-6.
The molecular types of lecithin in beef heart lipids were investigated by a combination of mild hydrolytic procedures and silicic acid chromatography. The major species of sn-glycero-3-phosphorylcholine (GPC) were found to be: diacyl-GPC (57%), alk-l-enyl, acyl-GPC (39%), alkyl acyl-GPC (3%), and dialkyl-GPC ( less than 1%). No di-alk-l-enyl-GPC or alk-l-enyl alkyl-GPC were detected. The derived monoalkyl-and dialkyl-glycerols were characterized by their infrared spectra and alkyl chain compositions.
通过温和水解程序和硅酸色谱法相结合的方法，对牛心脂质中卵磷脂的分子类型进行了研究。发现sn-甘油-3-磷酸胆碱（GPC）的主要种类为：二酰基-GPC（57%）、1-烯基酰基-GPC（39%）、烷基酰基-GPC（3%）和二烷基-GPC（小于1%）。未检测到二-1-烯基-GPC或1-烯基烷基-GPC。通过红外光谱和烷基链组成对衍生的单烷基甘油和二烷基甘油进行了表征。
